Amyloid beta (1-40/42) Mouse Monoclonal Antibody [Clone ID: 9F1]

SKU AM00002PU-N

Amyloid beta (1-40/42) mouse monoclonal antibody, clone 9F1, Purified

Product Data

• Clone Name: 9F1
• Application: ELISA, IF, WB
• Recommended Dilution: Western Blot: 1 μg/ml for HRPO/ECL detection.; Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.; ELISA: 0.05 μg/ml.; Immunocytochemistry: 0.1-1 μg/ml.
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: KLH conjugated C-terminal peptide of Amyloid beta A4 (1-40) and beta A4 (1-42).
• Specificity: This antibody interacts with the C-termini of both beta-Amyloid (1-40) and (1-42).
• Buffer: 1 ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose; State: Purified; State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -20°C to -80°C.; Avoid repeated freezing and thawing.; Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Shipping: Ambient
• Background: The beta-amyloid peptide (beta A4), proteolytically released from the amyloid precursor protein (APP), is the principal component of senile plaques in Alzheimer's disease. Cleavage of APP by alpha-secretase or alternatively by beta-secretase leads to generation and extracellular release of soluble APP peptides, S-APP-alpha and S-APP-beta, respectively, and the retention of corresponding membrane-anchored C-terminal fragments, C83 and C99. Subsequent processing of C83 by gamma-secretase yields P3 peptides. This is the major secretory pathway and is nonamyloidogenic. Alternatively, presenilin/nicastrin-mediated gamma-secretase processing of C99 releases the amyloid beta proteins, amyloid-beta 40 (Abeta40) and amyloid-beta 42 (Abeta42), major components of amyloid plaques, and the cytotoxic C-terminal fragments, gamma-CTF(50), gamma-CTF(57) and gamma-CTF(59).

Reference Data