Mouse Antibody Used On Mouse Tissue
Antigen detection with primary antibody of the same species as the test tissue yields high background when indirect detection method is used. This severely limits the use of murine monoclonal antibody on mouse tissues. GBI Labs Klear mouse kit is designed for staining mouse antibodies on mouse tissues. The Klear Mouse HRP or AP-Polymer Detection kit uses a special blocking buffer, polymeric HRP or AP-linked secondary antibody as well as mouse antibody enhancer to detect mouse primary antibody that bound to the mouse tissue. This technology provides excellent sensitivity and high specificity. It is a biotin-free system, therefore, overcomes the non-specific staining caused by streptavidin/biotin system due to endogenous biotins. With Klear mouse system, the primary antibody will need to be screened at higher concentration (2-10 folds) than regular mouse detection kits.
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