Fluorescent Protein Antibodies

Immunoblot analysis of four different Myc-DDK tagged over-expression lysates and one Empty vector transfected 293T cells with TA150121 at 1:500.

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150028).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mGFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mGFP/mCFP/mYFP/KillerRed (TA180076, 1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY tdTomato (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tdTomato (TA180009, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dendra2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dendra2 (TA180094, 1:500).

HEK293T cells were transfected with the pCMV6- ENTRY tdTomato cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.5ug, 1ug of lysates (lane 1 to lane 4 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit tdTomato polyclonal antibody (TA150128) at 1:10000

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZsGreen1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZsGreen1 (TA180002, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY eGFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with 5A2 anti-eGFP monoclonal antibody (TA150052) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY turboGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-turboGFP polyclonal antibody (TA150071) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY turboGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-turboGFP polyclonal antibody (TA150075) (1:3000).

Anti-tRFP immunoblot analysis of HEK293T over-expression lysates containing recombinant tRFP, TurboFP602 TurboFP635 fluorescence proteins at 1:2000 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.2ug, 0.4ug, 0.8ug, 1.6ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit mCherry polyclonal antibody (TA150125) at 1:10000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRFP (TA180091, 1:500).

HEK293T cells were transfected with the pCMV6-ENTRY mKate (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-mKate polyclonal antibody (TA150072) (1:2000).

Hela cells transiently transfected with Paxillin-GFP (RG225997) were detached and seeded onto 0.2% gelatin coated coverslip. After 24 hours incubation, the cells were fixed with 4% formaldehyde for 30 min, permeabilized with 0.1% trixon-X100, and then stained with Dylight594-labeled tGFP antibodies (TA150024) and DAPI for nucleus counter staining. (a: Paxillin-GFP; b: anti-tGFP-DyLight594; c: Merged)

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mCherry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mCherry (TA180028, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (left lane) or pCMV6-ENTRY tYFP (right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tYFP (TA150027).

HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.5ug, 1ug, 2ug, 4ug, 8ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Chicken mCherry polyclonal antibody (TA150127) at 1:5000.

HEK293T cells were transfected with the pCMV6-ENTRY mCherry cDNA and the cell lysates were collected after 48 hours. 0ug, 0.5ug, 1ug, 2ug, 4ug, 8ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Goat mCherry polyclonal antibody (TA150126) at 1:5000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mOrange (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mOrange/mOrange2 (TA180053, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eCFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eCFP (TA180069, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY mGFP cDNA and the cell lysates were collected after 48 hours. 0ug, 0.1ug, 0.2ug, 0.4ug, 0.8ug, 1.6ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Rabbit mGFP polyclonal antibody (TA150122) at 1:5000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AcGFP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AcGFP1/PS-CFP2 (TA180011, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRFP (TA180093, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY tdTomato cDNA and the cell lysates were collected after 48 hours. 0ug, 0.5ug, 1ug, 2ug, 4ug, 8ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Goat tdTomato polyclonal antibody (TA150129) at 1:5000.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed2 (TA180005, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY eGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-eGFP polyclonal antibody (TA150070) (1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY mGFP cDNA and the cell lysates were collected after 48 hours. 0ug, 0.5ug, 1ug, 2ug, 4ug, 8ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Chicken mGFP polyclonal antibody (TA150124) at 1:3000.

HEK293T cells were transfected with the pCMV6-ENTRY mKate (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-mKate polyclonal antibody (TA150076) (1:3000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed-Express2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed-Express2 (TA180014, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DsRed-Monomer (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DsRed-Monomer (TA180083, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mStrawberry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mStrawberry (TA180039, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZsYellow1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZsYellow1 (TA180004, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY E2-Crimson (JA1132-C07, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2-Crimson (1:500).

Western blot analysis of extracts from HEK293 cells transfected with C-terminal HA-tagged gene (lane 2, 3, 4, with 5, 10, 20uL from left to right) and negative control vector (lane 1) using anti-HA tag rabbit monoclonal antibody (TA591010) at 1:2000 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY eGFP (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-eGFP polyclonal antibody (TA150074) (1:3000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY E2-Crimson (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-E2-Crimson (TA180085, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY tdTomato (JB1016_D07, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-tdTomato (1:500).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mPlum (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mPlum (TA180072, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRaspberry (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRaspberry/AmCyan1 (TA180029, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or HA tagged LGALS3 cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HA.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mRFP (TA180092, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY eRFP (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-eRFP (TA180047, 1:2000).

Western blot analysis of extracts from HEK293 cells transfected with C-terminal HA-tagged gene (lane 2, 3, 4, with 5, 10, 20uL from left to right) and negative control vector (lane 1) using anti-HA tag rabbit monoclonal antibody (TA591011) at 1:2000 dilution.

HEK293T cells were transfected with the pCMV6-ENTRY mGFP cDNA and the cell lysates were collected after 48 hours. 0ug, 0.5ug, 1ug, 2ug, 4ug, 8ug of lysates (lane 1 to lane 6 respectively) were separated by SDS-PAGE and immunoblotted with Goat mGFP polyclonal antibody (TA150123) at 1:2000.

HEK293T cells were transfected with the pCMV6-ENTRY mPlum (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Rabbit anti-mPlum polyclonal antibody (TA150073) (1:2000).

HEK293T cells were transfected with pCMV6-Entry-AsRed2 cDNA for 48 hours. Serial dilution of AsRed2 expression lysates were separated by SDS-PAGE and immunoblotted with anti-AsRed2 (TA180099, 1:1000) (Lane 1, 5ug; Lane 2, 2.5ug; Lane 3, 1.25ug; Lane 4, 0.625ug; Lane 5, 0.3125ug).

HEK293T cells were transfected with the pCMV6-ENTRY mPlum (left lane) cDNA or pCMV6-ENTRY control (right lane) for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immuno-blotted with Chicken anti-mPlum polyclonal antibody (TA150077) (1:3000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Timer (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Timer (TA180007, 1:2000).

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mBanana (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-mBanana (TA180031, 1:2000).