pCMV6-AC-Myc-DDK-IRES-GFP-Puro Mammalian Expression Vector

Snippet: 2019S variant were described in [3]. The pCMV6-mGLT-1 (Myc-DDK-tagged) plasmid encoding the mouse glial high-affinity glutamate transporter member 2 (Slc1a2), transcript variant 1, was purchased from OriGene Technologies Inc (Cat. MR226166). For the pEGFP-rat Glt1 plasmid, the 99–1820 bp fragment of the Rat Glt-1 plasmid (GenBankTM accession number X67857.1) was subcloned between the EcoRI and

Snippet: s 100%. Establishment of H-2Kb-expressing LLC cells The H-2Kb cDNA was synthesized from mRNA of C57BL/6 mouse spleen and inserted into the pCMV6-AC-Myc-DDK-IRES-GFP-Puro Mammalian Expression Vector (ORIGENE, USA, Rockville, MD, USA). LLC cells, purchased from American Type Culture Collection, were transfected with pCMV6-AC-Myc-DDK-IRES-GFP-Puro-H-2Kb by Lipofectamine 3000 Reagent (Thermo Fisher S...

Snippet: E2-TM4-C3 (AA 113-249), TM4-C3 (AA 214-249) and C3 (AA 235-249) tetraspanin 7 fragments NH2-terminally fused to NanoLuc were constructed by PCR amplification from full-length tetraspanin 7 [7] and cloning into pCMV6-AC-IRES-GFP-Puro (Origene, Rockville

Snippet: 2.2. Cloning and expression of antigens. cDNA from human peripheral mononuclear cells was used to clone full length NUP50 and MTIF3 into pCMV6-AC-IRES- GFP-Puro (Origene, MD, USA) containing NanoLuc Luciferase

Snippet: The PCR 95 fragments were cloned into pCMV6 - AC - I RES - GFP - Puro vector (OriGene)