This vector is essentially the same as the pCMV6-Entry vector, except for the alternative selection marker (GFP replacing Neomycin). GFP can be used to monitor transfection efficiency or as a cell sorting marker.
TurboGFP (excitation/ emission max = 482/ 502 nm) is mainly intended for applications where fast appearance of bright fluorescence is crucial. It is specially recommended for cell and organelle labeling and tracking the promoter activity. Structually it forms dimer. Learn more about TurboGFP
There is no drug selection marker in this vector for mammalian cells.
The vector itself does not contain additional tag. If the insert is shuttled from pCMV6-Entry via SgfI/AscI and RsrII/MluI, it will express non-tagged protein. If the insert is shuttled from the pCMV6-Entry vector using SgfI/AscI and Pme I, then it will express Myc-DDK tagged protein.
V2_F Primer can be used to sequence the target sequence after cloning.
XL39_R Primer can be used to sequence the target sequence after cloning.
Forward primer to sequence targets cloned in expression vectors.
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