pLenti-PGK-C-mGFP-P2A-Puro Lentiviral Gene Expression Vector
pLenti-PGK-C-mGFP-P2A-Puro, Lenti vector with PGK promoter, C-terminal mGFP tag and P2A-Puro, 10ug
E. coli Selection
This Lenti vector is a third generation lentiviral vector.
The ORF cloned in this vector can be expressed as a tagged fusion protein with C-terminal mGFP tag. The expression of the cloned ORF is driven by PGK promoter. P2A-Puro is within the Lentiviral packaging cassette and would allow puromycin selection in both the transfected and the transduced cells. A brief lentiviral packaging protocol is provided. E. Coli. selection: Chloramphenicol (34ug/ml) All OriGene's TrueORF clone can be shuttled into this vector via
PrecisionShuttling, a simple cut-and-ligation process.
PGK_F Primer can be used to sequence the target sequence after cloning.
mGFP_R Primer can be used to sequence the target sequence after cloning. Download full vector sequence
Schematic of the multiple cloning sites
TurboFectin Transfection Reagent (1 mL in 1 vial)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks