GTP cyclohydrolase 1 (GCH1) Human shRNA Plasmid Kit (Locus ID 2643)
GCH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector
|Synonyms||DYT5; DYT5a; DYT14; GCH; GTP-CH-1; GTPCH1; HPABH4B|
|E. coli Selection||Ampicillin|
|Mammalian Cell Selection||Puromycin|
|Kit Components||GCH1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 2643). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.|
|RefSeq||NM_000161, NM_001024024, NM_001024070, NM_001024071, NM_000161.1, NM_000161.2, NM_001024071.1, NM_001024070.1, NM_001024024.1, BC025415, BM971258, NM_001024070.2, NM_001024071.2, NM_000161.3|
|Summary||This gene encodes a member of the GTP cyclohydrolase family. The encoded protein is the first and rate-limiting enzyme in tetrahydrobiopterin (BH4) biosynthesis, catalyzing the conversion of GTP into 7,8-dihydroneopterin triphosphate. BH4 is an essential cofactor required by aromatic amino acid hydroxylases as well as nitric oxide synthases. Mutations in this gene are associated with malignant hyperphenylalaninemia and dopa-responsive dystonia. Several alternatively spliced transcript variants encoding different isoforms have been described; however, not all variants give rise to a functional enzyme. [provided by RefSeq, Jul 2008]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact email@example.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).