RPA34 (RPA2) Human shRNA Plasmid Kit (Locus ID 6118)

SKU TR309766

RPA2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector, 5µg of each construct provided

Product Data

• Locus ID: 6118
• Synonyms: REPA2; RP-A p32; RP-A p34; RPA32
• Vector: pRS
• E. coli Selection: Ampicillin
• Format: Retroviral plasmids
• Components: RPA2 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6118). 5µg purified plasmid DNA per construct; 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.
• RefSeq: NM_001286076, NM_001297558, NM_002946, NM_001355128, NM_001355129, NM_002946.1, NM_002946.2, NM_002946.3, NM_002946.4, NM_001286076.1, BC021257, BC021257.2, BC001630, BC012157, NM_002946.5
• UniProt ID: P15927
• Summary: This gene encodes a subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding (OB) domains of the complex are utilized, and differing in the length of DNA bound. This subunit contains a single OB domain that participates in high-affinity DNA binding and also contains a winged helix domain at its carboxy terminus, which interacts with many genome maintenance protein. Post-translational modifications of the RPA complex also plays a role in co-ordinating different damage response pathways. [provided by RefSeq, Sep 2017]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Shipping: Ambient