Stromal interaction molecule 1 (STIM1) Human shRNA Plasmid Kit (Locus ID 6786)
STIM1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector
|Product Name||Stromal interaction molecule 1 (STIM1) Human shRNA Plasmid Kit (Locus ID 6786)|
|Synonyms||D11S4896E; GOK; IMD10; STRMK; TAM; TAM1|
|E. coli Selection||Ampicillin|
|Kit Components||STIM1 - Human, 4 unique 29mer shRNA constructs in retroviral untagged vector(Gene ID = 6786). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pRS Vector, TR30012, included for free.|
|RefSeq||NM_001277961, NM_001277962, NM_003156, BC021300, BC019905, BM127091|
|Summary||This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact email@example.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
STIM1 and STIM2 Proteins Differently Regulate Endogenous Store-operated Channels in HEK293 Cells
,Shalygin, A;Skopin, A;Kalinina, V;Zimina, O;Glushankova, L;Mozhayeva, GN;Kaznacheyeva, E;,
J. Biol. Chem. Dec 2014
,PubMed ID 25533457
JBC Papers in Press. Published on December 22, 2014 as Manuscript M114. 601856
,Shalygin, A;Skopin, A;Kalinina, V;Zimina, O;,
The Journal of Biological Chemistry Dec 2014
Lipid rafts couple store-operated Ca2+ entry to constitutive activation of PKB/Akt in a Ca2+/calmodulin-, Src- and PP2A-mediated pathway and promote melanoma tumor growth
,Shlomit Fedida-Metula, Ben Feldman, Valeria Koshelev, Uliana Levin-Gromiko, Elena Voronov, and Daniel Fishman,
Carcinogenesis, Feb 2012; 10.1093/carcin/bgs021.
Evidence for STIM1- and Orai1-dependent store-operated calcium influx through ICRAC in vascular smooth muscle cells: role in proliferation and migration
,Marie Potier, José C. Gonzalez, Rajender K. Motiani, Iskandar F. Abdullaev, Jonathan M. Bisaillon, Harold A. Singer, and Mohamed Trebak,
FASEB J, Apr 2009; 10.1096/fj.09-131128.
Store-operated cyclic AMP signalling mediated by STIM1
,Konstantinos Lefkimmiatis, Meera Srikanthan, Isabella Maiellaro, Mary Pat Moyer, Silvana Curci, Aldebaran M. Hofer,
Nature Cell Biology 11, 433-442 (15 March 2009)
Canonical Transient Receptor Potential 5 Channel in Conjunction with Orai1 and STIM1 Allows Sr2+ Entry, Optimal Influx of Ca2+, and Degranulation in a Rat Mast Cell Line
,Hong-Tao Ma, Ze Peng, Takaaki Hiragun, Shoko Iwaki, Alasdair M. Gilfillan, and Michael A. Beaven,
J. Immunol., Feb 2008; 180: 2233 - 2239.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.