Aryl hydrocarbon Receptor (AHR) Human shRNA Plasmid Kit (Locus ID 196)

CAT#: TL320259

AHR - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector



USD 1,013.00

In Stock*

Size
    • 5 ug/vial

Frequently bought together (2)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

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TurboFectin Transfection Reagent (1 mL in 1 vial)
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Specifications

Product Data
Locus ID 196
Synonyms bHLHe76; RP85
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Kit Components AHR - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 196). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001621, NM_001621.1, NM_001621.2, NM_001621.3, NM_001621.4, BC070080, BC070080.1, BC021696, BC069390, NM_001621.5
UniProt ID P35869
Summary The protein encoded by this gene is a ligand-activated helix-loop-helix transcription factor involved in the regulation of biological responses to planar aromatic hydrocarbons. This receptor has been shown to regulate xenobiotic-metabolizing enzymes such as cytochrome P450. Before ligand binding, the encoded protein is sequestered in the cytoplasm; upon ligand binding, this protein moves to the nucleus and stimulates transcription of target genes. [provided by RefSeq, Sep 2015]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.