Sodium Potassium ATPase (ATP1A1) Human shRNA Plasmid Kit (Locus ID 476)

CAT#: TL314602

ATP1A1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided

Product Images

GFP signal was observed under microscope at 48 hours after transduction of [TL314602C] virus into HEK293 cells. [TL314602C] virus was prepared using lenti-shRNA [TL314602C] and TR30037 packaging kit.

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  GFP signal was observed under microscope at 48 hours after transduction of [TL314602D] virus into HEK293 cells. [TL314602D] virus was prepared using lenti-shRNA [TL314602D] and TR30037 packaging kit.

Specifications

Product Data

• Locus ID: 476
• Synonyms: CMT2DD; HOMGSMR2
• Vector: pGFP-C-shLenti
• E. coli Selection: Chloramphenicol (34 ug/ml)
• Mammalian Cell Selection: Puromycin
• Format: Lentiviral plasmids
• Kit Components: ATP1A1 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 476). 5µg purified plasmid DNA per construct
  29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• RefSeq: NM_000701, NM_001001586, NM_001160233, NM_001160234, NM_000701.1, NM_000701.2, NM_000701.3, NM_000701.4, NM_000701.5, NM_000701.6, NM_000701.7, NM_001160233.1, NM_001160234.1, NM_001001586.1, BC003077, BC003077.2, BC001330, BC050359
• UniProt ID: P05023
• Summary: The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]
• shRNA Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
  
  For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).