DGKA Human shRNA Plasmid Kit (Locus ID 1606)

CAT#: TL305017

DGKA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided


USD 1,013.00

2 Weeks*

Size
    • 1 kit

Product Images

Frequently bought together (4)
Lenti-vpak packaging kit - packaging plasmids and transfection reagent
    • 10 reactions

USD 695.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

USD 507.00


DGKA mouse monoclonal antibody, clone OTI3G7 (formerly 3G7)
    • 100 ul

USD 478.00


Lateral flow testing sticks used for the semi-quantitative detection of the lentiviral p24 protein, 20 tests
    • 20 Tests

USD 428.00

Other products for "DGKA"

Specifications

Product Data
Locus ID 1606
Synonyms DAGK; DAGK1; DGK-alpha
Vector pGFP-C-shLenti
E. coli Selection Chloramphenicol (34 ug/ml)
Mammalian Cell Selection Puromycin
Format Lentiviral plasmids
Kit Components DGKA - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 1606). 5µg purified plasmid DNA per construct
29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
RefSeq NM_001345, NM_201444, NM_201445, NM_201554, NM_001351033, NM_001351034, NM_001351035, NM_001351036, NM_001351037, NM_001351038, NM_001351039, NM_001351040, NR_147026, NR_147027, NM_201444.1, NM_201444.2, NM_201445.1, NM_201554.1, NM_001345.1, NM_001345.2, NM_001345.3, NM_001345.4, BC023523, BC023523.2, BC031870, BM836118, BM910598, NM_001345.5
UniProt ID P23743
Summary The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.