IDH2 Human shRNA Plasmid Kit (Locus ID 3418)
IDH2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||IDH2 Human shRNA Plasmid Kit (Locus ID 3418)|
|Synonyms||D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH|
|Kit Components||IDH2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 3418). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|RefSeq||NM_001289910, NM_001290114, NM_002168|
|Summary||Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the mitochondria. It plays a role in intermediary metabolism and energy production. This protein may tightly associate or interact with the pyruvate dehydrogenase complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Reductive carboxylation and 2-hydroxyglutarate formation by wild-type IDH2 in breast carcinoma cells
,Smolková, K;Dvorák, A;Zelenka, J;Vítek, L;Ježek, P;,
Int. J. Biochem. Cell Biol. May 2015,PubMed ID 26007236