NFE2L2 Human shRNA Plasmid Kit (Locus ID 4780)
NFE2L2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector
|Product Name||NFE2L2 Human shRNA Plasmid Kit (Locus ID 4780)|
|Kit Components||NFE2L2 - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 4780). 5µg purified plasmid DNA per constructNon-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.|
|RefSeq||NM_001145412, NM_001145413, NM_006164|
|Summary||This gene encodes a transcription factor which is a member of a small family of basic leucine zipper (bZIP) proteins. The encoded transcription factor regulates genes which contain antioxidant response elements (ARE) in their promoters; many of these genes encode proteins involved in response to injury and inflammation which includes the production of free radicals. Multiple transcript variants encoding different isoforms have been characterized for this gene. [provided by RefSeq, Sep 2015]|
|shRNA Design||These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, align it with our published shRNA design sequences. If these do not align, please utilize our custom shRNA service|
|Performance Guaranteed||OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.
For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at firstname.lastname@example.org. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
|The use of this RNAi has been cited in the following citations:|
Nuclear factor erythroid 2-related factor 2 enhances carcinogenesis by suppressing apoptosis and promoting autophagy in nickel-transformed cells
,Son, YO;Pratheeshkumar, P;Divya, SP;Zhang, Z;Shi, X;,
J. Biol. Chem 2017,PubMed ID 28330870
Different roles of ROS and Nrf2 in Cr(VI)-induced inflammatory responses in normal and Cr(VI)-transformed cells.
,Roy, RV;Pratheeshkumar, P;Son, YO;Wang, L;Hitron, JA;Divya, SP;Zhang, Z;Shi, X;,
Toxicol. Appl. Pharmacol. 2016,PubMed ID 27470422
Anti-oncogenic and oncogenic properties of Nrf2 in arsenic-induced carcinogenesis
,Son, YO;Pratheeshkumar, P;Roy, RV;Hitron, JA;Wang, L;Divya, SP;Xu, M;Luo, J;Chen, G;Zhang, Z;Shi, X;,
J. Biol. Chem. Sep 2015,PubMed ID 26385919
ROS/Autophagy/Nrf2 Pathway Mediated Low-Dose Radiation Induced Radio-Resistance in Human Lung Adenocarcinoma A549 Cell
,Chen, N;Wu, L;Yuan, H;Wang, J;,
Int. J. Biol. Sci. Jun 2015,PubMed ID 26078725