PGC1 alpha (PPARGC1A) Human shRNA Plasmid Kit (Locus ID 10891)

CAT#: TG310260

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PPARGC1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector



USD 825.00


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Size
    • 5 ug/vial


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Specifications

Product Data
Locus ID 10891
Synonyms LEM6; PGC-1(alpha); PGC-1alpha; PGC-1v; PGC1; PGC1A; PPARGC1
Vector pGFP-V-RS
E. coli Selection Kanamycin
Mammalian Cell Selection Puromycin
Format Retroviral plasmids
Kit Components PPARGC1A - Human, 4 unique 29mer shRNA constructs in retroviral GFP vector(Gene ID = 10891). 5µg purified plasmid DNA per construct
Non-effective 29-mer scrambled shRNA cassette in pGFP-V-RS Vector, TR30013, included for free.
RefSeq NM_001330751, NM_001330752, NM_001330753, NM_013261, NM_001354825, NM_001354826, NM_001354827, NM_001354828, NR_148981, NR_148982, NR_148983, NR_148984, NR_148985, NR_148986, NR_148987, NM_013261.1, NM_013261.2, NM_013261.3, NM_013261.4, BC029800, BC156323, BC157068, BM976883, NM_013261.5
UniProt ID Q9UBK2
Summary The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity. [provided by RefSeq, Jul 2008]
shRNA Design These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
Performance Guaranteed OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples.

For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
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