MIRLET7i Human MicroRNA Expression Plasmid (MI0000434)

SKU SC400011

Expression plasmid for human microRNA LET7i

Product Data

• Species: Human
• Vector: pCMV-MIR
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Mammalian Cell Selection:
  Precursor: CUGGCUGAGGUAGUAGUUUGUGCUGUUGGUCGGGUUGUGACAUUGCCCGCUGUGGAGAUAACUGCGCAAGCUACUGCCUUGCUA:
  Mature Sequence: UGAGGUAGUAGUUUGUGCUGUU:
  Full Insert

  >MI0000434 CGCCGCTTTTCTCTCTTCAGAGCGGTGGCTGACGTACAGAACGCCGGCTGTATCACCCGGGCGCCGGCGC; CGCCTCTCATCCCCGTAGCTCTCGGGGCGCGCCGGGAGTAGCCGAGCCGCTGGGTGCTGGGCGGGGTCTC; CGGGCGCGGCGCGGGGGGAAGGCAAAGGAAAAGAAGGGGCGGCGGGCCGACGTCGGCGGCGAGGAAGGAC; GGAGGAGCGCCGGAGCCGCTTTGCTGCCCTCCGCGTGGTCCCGTGCCTCCCCGACACCATGGCCCTGGCT; GAGGTAGTAGTTTGTGCTGTTGGTCGGGTTGTGACATTGCCCGCTGTGGAGATAACTGCGCAAGCTACTG; CCTTGCTAGTGCTGGTGATGCTCAGCGCCGCGGAGGACAATGGCTGGGAATCCCCTTTGTTTTCCGGGGC; GCTGCCTGGGGACAGCCCGCGAAGCCTCGGCGCCCGGCCGGCGCGGCCACCACGGAAACCGTTAGTTTCA; CATTTTCCTAGAAGGAATTGAGGGGAGTGGGGTGGACCGCGATGCATTTTAAAGGTTGGGAAAGAGCTAA; GCCATTGGATTCCTTTTCTTGAAGGAAGGAGTGGAATTGTGTAAGATAATTGATACAGATAACTTTTTCA; CCCTAAAA
• Components: 1 x 10 ug vial miRNA plasmid lyophilized in a 2-D bar-coded Matrix tube
• Also Contains: Both mature and minor (star) miRNA can be over expressed from the construct.
• OTI Disclaimer: All miRNA clones were sequenced to ensure that the pre-mir sequences match reference sequence in miRBase. The flanking sequence of a miRNA clone may differ from the NCBI reference with respect to biological polymorphisms. This should not affect the function of the mature miRNA.
• Shipping: Ambient

Reference Data

• RefSeq: MI0000434
• Synonyms: LET7I MIRNLET7I hsa-let-7i
• Locus ID: 406891