XPD (ERCC2) (NM_000400) Human Recombinant Protein

SKU TP762657

Purified recombinant protein of Human excision repair cross-complementing rodent repair deficiency, complementation group 2 (ERCC2), transcript variant 1, full length, with N-GST and C-His tag, expressed in E.coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region full length of ERCC2
• Tag: N-GST and C-HIS
• Predicted MW: 114.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C after receiving vials.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000391
• Locus ID: 2068
• UniProt ID: P18074
• Cytogenetics: 19q13.32
• RefSeq Size: 2355
• RefSeq ORF: 2280
• Synonyms: COFS2; EM9; TFIIH; TTD; TTD1; XPD
• Summary: The nucleotide excision repair pathway is a mechanism to repair damage to DNA. The protein encoded by this gene is involved in transcription-coupled nucleotide excision repair and is an integral member of the basal transcription factor BTF2/TFIIH complex. The gene product has ATP-dependent DNA helicase activity and belongs to the RAD3/XPD subfamily of helicases. Defects in this gene can result in three different disorders, the cancer-prone syndrome xeroderma pigmentosum complementation group D, trichothiodystrophy, and Cockayne syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair