Topoisomerase I (TOP1) (NM_003286) Human Recombinant Protein

SKU TP761924

Purified recombinant protein of Human topoisomerase (DNA) I (TOP1),Lys93-Lys151, with N-terminal His-ABP tag, expressed in E. coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region(Lys93-Lys151) of TOP1
• Tag: N-His-ABP (Albumin-Binding Protein)
• Predicted MW: 21.9 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_003277
• Locus ID: 7150
• UniProt ID: P11387
• Cytogenetics: 20q12
• RefSeq Size: 3734
• RefSeq ORF: 2295
• Synonyms: TOPI
• Summary: This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome