PKC eta (PRKCH) (NM_006255) Human Recombinant Protein

SKU TP761777

Purified recombinant protein of Human protein kinase C, eta (PRKCH), full length, with N-terminal GST and C-terminal His tag, expressed in E. coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length PRKCH
• Tag: N-GST and C-His
• Predicted MW: 105.6 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 8 M urea
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_006246
• Locus ID: 5583
• UniProt ID: P24723
• Cytogenetics: 14q23.1
• RefSeq Size: 3868
• RefSeq ORF: 2049
• Synonyms: nPKC-eta; PKC-L; PKCL; PRKCL
• Summary: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. Mutations in this gene are associated with susceptibility to cerebral infarction. [provided by RefSeq, Sep 2015]
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Tight junction, Vascular smooth muscle contraction