ALAS1 (NM_199166) Human Recombinant Protein

SKU TP760337

Purified recombinant protein of Homo sapiens aminolevulinate, delta-, synthase 1 (ALAS1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding human full-length ALAS1
• Tag: N-His
• Predicted MW: 70.4 kDa
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris-HCl, pH 8.0, 150 mM NaCl, 1% sarkosyl, 10% glycerol
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_954635
• Locus ID: 211
• UniProt ID: P13196
• Cytogenetics: 3p21.2
• RefSeq Size: 2281
• RefSeq ORF: 1920
• Synonyms: ALAS; ALAS-H; ALAS3; ALASH; MIG4
• Summary: This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2015]
• Protein Pathways: Glycine, Metabolic pathways, Porphyrin and chlorophyll metabolism, serine and threonine metabolism