Collagen XVII (COL17A1) (NM_000494) Human Recombinant Protein

SKU TP750243

Purified recombinant protein of 180 kDa bullous pemphigoid antigen 2 (BP180), Glu490-Gly567, with N-terminal His-GST tag, expressed in E.coli, 100ug

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Glu490-Gly567) of BP180
• Tag: N-terminal His-GST tag
• Predicted MW: 37.8 kDa
• Concentration: >100 ug/mL as determined by microplate BCA method
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 50 mM Tris-HCl, pH 8.0, 500 mM NaCl, 10% glycerol, 1% sarkosyl
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Stability: Stable for at least 1 year from receipt of products under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data

• RefSeq: NP_000485
• Locus ID: 1308
• UniProt ID: Q9UMD9
• Cytogenetics: 10q25.1
• RefSeq Size: 5610
• RefSeq ORF: 4491
• Synonyms: BA16H23.2; BP180; BPA-2; BPAG2; EBR2A; ERED; JEB-I; LAD-1
• Summary: This gene encodes the alpha chain of type XVII collagen. Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is a structural component of hemidesmosomes, multiprotein complexes at the dermal-epidermal basement membrane zone that mediate adhesion of keratinocytes to the underlying membrane. Mutations in this gene are associated with both generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimeric forms of type XVII collagen exist. The full length form is the transmembrane protein. A soluble form, referred to as either ectodomain or LAD-1, is generated by proteolytic processing of the full length form. [provided by RefSeq, Jul 2008]
• Protein Families: Transmembrane