FGF2 (NM_002006) Human Recombinant Protein

CAT#: TP750002

Recombinant protein of human Fibroblast Growth Factor-basic (FGF2) produced in E. coli

Product Images

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  Cell Proliferation Assay: 3T3 fibroblast cells were cultured in increasing concentration (0 to 10 ng/ml) of h-FGF-Basic growth factor and cell density was measured after 8.5 hours at 490 nm. The ED50 for this effect is typically 0.07-0.10 ng/ml.

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  A DNA sequence encoding the region (Gly132-Ser288) of human FGF2
• Tag: Tag Free
• Predicted MW: 17.4 kDa
• Concentration: >0.1 µg/µL as determined by microplate BCA method
• Purity: >95% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: Lyophilized from a sterile solution containing 10 mM Tris-Cl, pH 7.0, 150 mM NaCl
• Bioactivity: Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.07-0.10 ng/ml.
  Cell culture factor (PMID: 26253201)
  Cell culture factor (PMID: 26704449)
  Cell culture factor (PMID: 29416007)
  Cell culture factor (PMID: 29937202)
  Cell culture factor (PMID: 29948854)
• Endotoxin: < 0.1 EU per 1 µg of the protein by the LAL
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_001997
• Locus ID: 2247
• UniProt ID: P09038
• Cytogenetics: 4q28.1
• Refseq Size: 6803
• Refseq ORF: 864
• Synonyms: BFGF; FGF-2; FGFB; HBGF-2
• Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton