Escherichia coli Recombinant Protein

SKU TP724706

Recombinant E.coli RNA-splicing ligase RtcB

Product Data

• Species: Escherichia coli
• Protein Source: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  M1-G408
• Buffer: Supplied as a 0.2 um filtered solution of 20mM HEPES, 300mM NaCl,10%glycerol, 2mM DTT,0.1mM EDTA,pH7.5.
• Note: Recombinant E.coli RNA-splicing ligase RtcB is produced by E.coli. The target gene encoding M1-G408 is expressed.
• Storage: Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
• Stability: 12 months from date of despatch
• Shipping: Dry Ice

Reference Data

• Synonyms: 3'-phosphate/5'-hydroxy nucleic acid ligase; rtcB; yhgL
• Summary: RNA-splicing ligase RtcB is a member of RtcB family. It is a GTP-dependent RNA ligase that is involved in tRNA splicing and RNA repair. RtcB rejoins broken stem-loops of tRNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends. RtcB can also act as a DNA ligase in case of DNA damage by splicing 'dirty' DNA breaks, characterized by 3'-phosphate (or cyclic-phosphate) and 5'-hydroxy ends that cannot be sealed by classical DNA ligases.