S Protein SARS-CoV-2 Recombinant Protein

CAT#: TP723959

SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag

Product Images

Figure 1. SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag on SDS-PAGE under reducing condition.

• 

  Figure 2. ELISA plate pre-coated by 2 µg/ml (100 µl/well) SARS-CoV-2 (2019-nCoV) S protein RBD, His tagged protein (TP723959) can bind its native ligand Human ACE2, hFc tagged protein (TP723981) in a linear range of 1.96-31.72 ng/ml.

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  S protein RBD（Arg319-Phe541）+6×His tag
• Tag: C-6×His
• Predicted MW: 37-38 kDa
• Purity: > 95%
• Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose is added as protectants before lyophilization
• Reconstitution: Reconstitute with deionized water
• Preparation: Affinity purification
• Applications: ELISA
• Storage: Store the lyophilized protein at -20°C.
  After reconstitution, store the protein at -80°C for 12 months.
  Avoid repeated freezing and thawing.
• Stability: 12 months from date of despatch

Reference Data

• Locus ID: 43740568
• Summary: SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as Covid19 (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein is a type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which accounts for recognizing the cell surface receptor, ACE2. S2 contains basic elements needed for the membrane fusion. Recent publications indicate that S1-RBD domain can induce virus neutralizing-antibody and T cell response.