SARS-CoV-2 Spike S1 Recombinant Protein with His tag

CAT#: TP723919

SARS-CoV-2 Spike S1 Recombinant Protein with His tag

Product Images

Recombinant SARS-COV-2 Spike S1 Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 110-130 kDa.

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  The purity of SARS-COV-2 Spike S1 Protein with His tag (Cat.TP723919) was greater than 90% as determined by SEC-HPLC.

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  Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 27.3 nM as determined in a SPR assay (Nicoya OpenSPR).

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  Immobilized SARS-CoV-2 Spike S1 at 2μg/mL (100 μL/well) can bind recombinant Human ACE2 with a linear range of 0.03-7.12 ng/mL.

Specifications

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PRR
• Tag: C-6xHis
• Purity: > 90% by SDS-PAGE.
• Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Endotoxin: < 1.0 EU/μg of the protein by LAL method.
• Protein Description: Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg683) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.
• Storage: Store at -20°C to -80°C.
• Stability: 3 months

Reference Data

• Locus ID: 43740568