FGF2 (NM_002006) Human Recombinant Protein

CAT#: TP723719

Purified recombinant protein of Human fibroblast growth factor 2 (basic) (FGF2)

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Human FGF-basic, the region of Ala144-Ser288, from gene Accession# NM_002006
• Tag: Tag Free
• Predicted MW: 16.3 kDa
• Concentration: lot specific
• Purity: Purity is >98%, as determined by Coomassie stained SDS-PAGE.
• Buffer: 10 mM NaH2PO4, 150 mM NaCl, pH 7.2, 1 mM DTT
• Bioactivity: The ED50 is 1 - 4 ng/ml, corresponding to a specific activity of 1 - 0.25 x 10^6 units/mg, determined by the dose dependent stimulation of NIH/ 3T3 cell proliferation. The bioactivity is equivalent to competitor reported values.
• Endotoxin: Less than 0.01 ng per ug protein as determined by the LAL method
• Storage: Store at -80°C.
• Stability: Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to 6 months, or at -70°C or below until the expiration date. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.

Reference Data

• RefSeq: NP_001997
• Locus ID: 2247
• UniProt ID: P09038
• Cytogenetics: 4q28.1
• Refseq Size: 6803
• Refseq ORF: 864
• Synonyms: BFGF; FGF-2; FGFB; HBGF-2
• Summary: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton