CD16 (FCGR3A) Human Recombinant Protein

SKU TP721249M

APC Conjugated Human CD16a Protein (C-His, 176V)

Product Data

• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  Gly17-Gln208
• Tag: C-His
• Predicted MW: The protein has the predicted molecular weight of 24 kDa and migrates at approximately 40-50 kDa on SDS-PAGE with DTT-reduced condition before APC conjugation.
• Concentration: 25µg size is bottled at 0.1mg/mL concentration. 100 µg size is bottled at lot specific concentration.
• Purity: >90%
• Conjugation: APC
• Presentation State: Purified
• Buffer: 1xPBS buffer, pH7.4, 0.09% NaN3 with a carrier protein
• Bioactivity: Positive; ; The definition of the active protein (purified and biotinylated) is defined as the protein that can bind to its biological receptor/ligand. For conjugated protein, it is defined with its function to bind to the ScFv of the active CAR-transfected cells in flow cytometry test.
• Preparation: Affinity Ni-NTA
• Recommended Dilution: FACS
• Storage: An unopened vial can be stored at 4°C for 2 weeks or at -20°C and below for six months. This stock solution should be aliquoted and stored at ≤ -70°C to minimize the freeze/thaw cycles.
• Stability: 6 Months
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_001121068
• Locus ID: 2214
• UniProt ID: P08637
• Protein Families: FACS
• Protein Pathways: Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, Systemic lupus erythematosus