SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein (Bioactivity Verified)

CAT#: TP701119

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Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag


Product Datasheet for 'TP701119'


USD 788.00


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Size
    • 50 ug


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Specifications

Product Data
Description Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag
Species SARS-CoV-2
Expression Host HEK293
Expression cDNA Clone or AA Sequence MALWIDRMQL LSCIALSLAL VTNSAKGIYQ TSNFRVQPTE SIVRFPNITN LCPFGEVFNA
TRFASVYAWN RKRISNCVAD YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG
DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF
ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV
CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLGGGS GGGSMADSNG TITVEELKKH
HHHHH
Tag C-HIS
Predicted MW 34 kDa
Concentration >50 ug/mL as determined by Bradford protein assay method.
Purity > 90% as determined by SDS-PAGE and Coomassie blue staining
Buffer PBS, 10% glycerol.
Bioactivity Binding of SARS-CoV-2 S-M Fusion Protein to Human ACE2: 2 ug/ml of SARS-CoV-2 Spike and Membrane viral fusion protein (gel insert) was immobilized on an ELISA plate and its ability to bind to Human ACE2 was measured. The EC50 was between 10-20 ng/ml.
Storage Store at -80°C.
Stability Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Reference Data

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