SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein (Bioactivity Verified)

SKU TP701119

Purified recombinant fusion protein that includes the RBD of SARS-CoV-2 Spike protein (S-Protein) and Virion Surface Domain of Membrane Protein (M-protein) with C-terminal HIS-tag

Product Data

• Species: SARS-CoV-2
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MALWIDRMQL LSCIALSLAL VTNSAKGIYQ TSNFRVQPTE SIVRFPNITN LCPFGEVFNA; TRFASVYAWN RKRISNCVAD YSVLYNSASF STFKCYGVSP TKLNDLCFTN VYADSFVIRG; DEVRQIAPGQ TGKIADYNYK LPDDFTGCVI AWNSNNLDSK VGGNYNYLYR LFRKSNLKPF; ERDISTEIYQ AGSTPCNGVE GFNCYFPLQS YGFQPTNGVG YQPYRVVVLS FELLHAPATV; CGPKKSTNLV KNKCVNFNFN GLTGTGVLTE SNKKFLGGGS GGGSMADSNG TITVEELKKH; HHHHH
• Tag: C-HIS
• Predicted MW: 34 kDa
• Concentration: >0.05 µg/µL as determined by Bradford protein assay method.
• Purity: > 90% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: PBS, 10% glycerol
• Bioactivity: Binding of SARS-CoV-2 S-M Fusion Protein to Human ACE2: 2 ug/ml of SARS-CoV-2 Spike and Membrane viral fusion protein (gel insert) was immobilized on an ELISA plate and its ability to bind to Human ACE2 was measured. The EC50 was between 10-20 ng/ml.
• Note: For testing in cell culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Shipping: Dry Ice

Reference Data