MTOR (NM_004958) Human Recombinant Protein

CAT#: TP320457

Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR)

Bulk Request/Custom Protein Development


 Product Datasheet for 'TP320457'

USD 680.00


Availability*
In Stock

Size
    • 20 ug

Product images

Specifications

Product Data
Description Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence Recombinant protein was produced with TrueORF clone, RC220457. Click on the TrueORF clone link to view cDNA and protein sequences.
Tag C-MYC/DDK
Predicted MW 288.7 kDa
Concentration >50 ug/mL as determined by microplate BCA method
Purity > 80% as determined by SDS-PAGE and Coomassie blue staining
Buffer 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.
Bioactivity MTOR activity verified in a biochemical assay: MTOR (mechanistic target of rapamycin) (TP320457) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. MTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Varying concentrations of MTOR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.

MTOR activity was measured using the HTRF KinEASE® assay from Cis-Bio International.
Reference Data
RefSeq NP_004949
Locus ID 2475
Refseq Size 8680
Cytogenetics 1p36.22
Refseq ORF 7647
Synonyms FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS
Summary The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008].
Protein Families
Protein Pathways
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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