MTOR (NM_004958) Human Recombinant Protein
Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR)
Product Datasheet for 'TP320457'
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|Description||Recombinant protein of human FK506 binding protein 12-rapamycin associated protein 1 (FRAP1/MTOR)|
|Expression cDNA Clone or AA Sequence||Recombinant protein was produced with TrueORF clone, RC220457. Click on the TrueORF clone link to view cDNA and protein sequences.|
|Predicted MW||288.7 kDa|
|Concentration||>50 ug/mL as determined by microplate BCA method|
|Purity||> 80% as determined by SDS-PAGE and Coomassie blue staining|
|Buffer||25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. Store at –80C. Avoid repeated freeze-thaw cycles. Stable for 3 months from receipt of products under proper storage and handling conditions.|
|Bioactivity||MTOR activity verified in a biochemical assay: MTOR (mechanistic target of rapamycin) (TP320457) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. MTOR is a serine/threonine protein kinase that regulates cell growth, cell proliferation, cell motility, cell survival, protein synthesis, and transcription. MTOR belongs to the phosphatidylinositol 3-kinase-related kinase protein family. Varying concentrations of MTOR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors.
MTOR activity was measured using the HTRF KinEASE® assay from Cis-Bio International.
|Synonyms||FRAP; FRAP1; FRAP2; RAFT1; RAPT1; SKS|
|Summary||The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq, Sep 2008].|
|Protein Families||Protein Kinase, Druggable Genome|
|Protein Pathways||ErbB signaling pathway, mTOR signaling pathway, Insulin signaling pathway, Adipocytokine signaling pathway, Type II diabetes mellitus, Pathways in cancer, Glioma, Prostate cancer, Acute myeloid leukemia|
|The use of this Proteins has been cited in the following citations:|
mTORC2 promotes type I insulin-like growth factor receptor and insulin receptor activation through the tyrosine kinase activity of mTOR
,Yin, Y;Hua, H;Li, M;Liu, S;Kong, Q;Shao, T;Wang, J;Luo, Y;Wang, Q;Luo, T;Jiang, Y;,
Cell Res. Nov 2015
,PubMed ID 26584640