RTF2 (NM_016407) Human Recombinant Protein

CAT#: TP301652M

Recombinant protein of human chromosome 20 open reading frame 43 (C20orf43)

Product Images

Coomassie blue staining of purified RTF2 protein (Cat# TP301652). The protein was produced from HEK293T cells transfected with RTF2 cDNA clone (Cat# RC201652) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Species: Human
• Expression Host: HEK293T
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  >RC201652 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGCDGGTIPKRHELVKGPKKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKS
  AEKALGKAASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLRCC
  GCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKKTKKPKAAESVSK
  PDVSEEAPGPSKVKTGKPEEASLDSREKKTNLAPKSTAMNESSSGKAGKPPCGATKRSIADSEESEAYKS
  LFTTHSSAKRSKEESAHWVTHTSYCF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Tag: C-Myc/DDK
• Predicted MW: 33.7 kDa
• Concentration: >50 ug/mL as determined by microplate BCA method
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Applications: Cell culture: For culture applications, please filter before use. Note that you may experience some loss of protein during the filtration process.
• Storage: Store at -80°C.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Reference Data

• RefSeq: NP_057491
• Locus ID: 51507
• UniProt ID: Q9BY42
• Cytogenetics: 20q13.31
• Refseq Size: 1672
• Refseq ORF: 918
• Synonyms: C20orf43; CDAO5; HSPC164; RTFDC1; SHUJUN-3
• Summary: Replication termination factor which is a component of the elongating replisome (Probable). Required for ATR pathway signaling upon DNA damage and has a positive activity during DNA replication. Might function to facilitate fork pausing at replication fork barriers like the rDNA. May be globally required to stimulate ATR signaling after the fork stalls or encounters a lesion (Probable). Interacts with nascent DNA (PubMed:29290612).[UniProtKB/Swiss-Prot Function]