Hepatocyte growth factor / HGF Human Protein

SKU DA3506

Hepatocyte growth factor / HGF human recombinant protein, 5 µg

Product Data

• Species: Human
• Expression Host: Insect
• Predicted MW: 78 kDa
• Concentration: N/A
• Purity: >95% pure by SDS-PAGE and visualised by silver stain.
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized without preservatives.; Buffer System: 50 mM Acetic Acid with BSA as stabilizer.
• Bioactivity: Biological: Human HGF is fully biologically active when compared to standards.; The activity was assayed for scattering activity in the MDCK cell assay. The ED50 was for this effect is typically at 0.5-1.0 ng/ml.
• Endotoxin: < 0.1 ng per µg of HGF
• Reconstitution Method: The lyophilized Human HGF is soluble in Acetic Acid (50 mM) and can be restored to a concentration of 100 µg/ml. Further dilutions should be made into buffer containing protein or medium containing serum.
• Preparation: Lyophilized without preservatives.
• Protein Description: Recombinant human HGF is a 78.0 kDa protein.
• Storage: The lyophilized protein is stable at -20°C for one year from despatch.; Reconstituted human HGF is stable for three months when stored in working aliquots; at -20°C.; Avoid repeated freezing and thawing.
• Shipping: Ambient

Reference Data

• RefSeq: NP_000592
• Locus ID: 3082
• UniProt ID: P14210
• Cytogenetics: 7q21.11
• Synonyms: Scatter factor, Hepatopoeitin-A, HPTA
• Summary: This gene encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate alpha and beta chains, which form the mature heterodimer. This protein is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. This protein also plays a role in angiogenesis, tumorogenesis, and tissue regeneration. Although the encoded protein is a member of the peptidase S1 family of serine proteases, it lacks peptidase activity. Mutations in this gene are associated with nonsyndromic hearing loss. [provided by RefSeq, Nov 2015]
• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Protease, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Melanoma, Pathways in cancer, Renal cell carcinoma