Glyoxalase I / GLO1 (1-184, His-tag) Mouse Protein

SKU AR52069PU-N

Glyoxalase I / GLO1 (1-184, His-tag) mouse protein, 50 µg

Product Data

• Species: Mouse
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAEPQPASSG LTDETAFSCC SDPDPSTKDF LLQQTMLRIK DPKKSLDFYT RVLGLTLLQK LDFPAMKFSL YFLAYEDKND IPKDKSEKTA WTFSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKI ATIILEHHHH HH
• Tag: His-tag
• Predicted MW: 21.8 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Liquid purified protein; Buffer System: Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Bioactivity: Specific: Specific activity is > 210 units/mg, and is defined as the amount of enzyme that will form 1.0 µmol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 6.5 at 25C.
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_001107032
• Locus ID: 109801
• UniProt ID: Q9CPU0
• Cytogenetics: 17 15.69 cM
• Synonyms: Methylglyoxalase, Aldoketomutase, Glx I, Ketone-aldehyde mutase
• Summary: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B. Required for normal osteoclastogenesis.[UniProtKB/Swiss-Prot Function]