UCHL1 / PGP9.5 (1-223) (recombinant) Human Protein

SKU AR50009PU-S

UCHL1 / PGP9.5 (1-223) (recombinant) human recombinant protein, 0.1 mg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
• Predicted MW: 24.8 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Liquid purified protein; Buffer System: 20 mM Tris-HCl pH 8.0, 2 mM EDTA
• Bioactivity: Specific activity is > 150pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37°C.
• Preparation: Liquid purified protein
• Recommended Dilution: Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.)

  2. Add 50ul of 1uM Ubiquitin-AMC.

  3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. ; - Ubiquitin-AMC (Enzo, Cat.No, BML-SE211); - 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077) ; - Fluorescent plate reader (PerkinElmer, VICTOR X3)

• Protein Description: Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_004172
• Locus ID: 7345
• UniProt ID: P09936
• Cytogenetics: 4p13
• Synonyms: UCH-L1, PGP 9.5, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5
• Summary: The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
• Protein Families: 0.1 mg/ml Ovalbumin, 0.5 mM EDTA, 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 1 mM DTT, 655077) <br/>- Fluorescent plate reader (PerkinElmer, black (greiner bio-one, BML-SE211)<br/>- 96 Well Polystyrene Microplate, Cat.No, pH 8.0.) <p/>2. Add 50ul of 1uM Ubiquitin-AMC. <p/>3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes. <br/>- Ubiquitin-AMC (Enzo, Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, VICTOR X3)
• Protein Pathways: Parkinson's disease