UCHL1 / PGP9.5 (1-223) (recombinant) Human Protein

CAT#: AR50009PU-N

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UCHL1 / PGP9.5 (1-223) (recombinant) human recombinant protein, 0.5 mg



USD 930.00


Availability*
3 Weeks

Size
    • 500 ug


Product images

Specifications

Product Data
Description UCHL1 / PGP9.5 (1-223) (recombinant) human recombinant protein, 0.5 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA
Predicted MW 24.8 kDa
Concentration lot specific
Purity >95% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl pH8.0, 2mM EDTA
Bioactivity Specific: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.
Preparation Liquid purified protein
Applications Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.)

2. Add 50ul of 1uM Ubiquitin-AMC.

3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo, Cat.No, BML-SE211)
- 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077)
- Fluorescent plate reader (PerkinElmer, VICTOR X3)

Protein Description Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_004172
Locus ID 7345
UniProt ID P09936, V9HW74
Cytogenetics 4p13
Synonyms HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1
Summary The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]
Protein Families Druggable Genome, Protease
Protein Pathways Parkinson's disease
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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