UCHL1 / PGP9.5 (1-223) (recombinant) Human Protein
UCHL1 / PGP9.5 (1-223) (recombinant) human recombinant protein, 0.5 mg
|Description||UCHL1 / PGP9.5 (1-223) (recombinant) human recombinant protein, 0.5 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA|
|Predicted MW||24.8 kDa|
|Purity||>95% by SDS - PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl pH8.0, 2mM EDTA
|Bioactivity||Specific: > 300 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C.|
|Preparation||Liquid purified protein|
|Applications||Protocol: 1. Prepare a 100ul of recombinant PGP9.5 protein with various concentrations (0.48ng, 0.9ng) in assay buffer and equilibrate to 37C for 10 minutes. (Assay buffer: 50mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 0.1 mg/ml Ovalbumin, pH 8.0.) 2. Add 50ul of 1uM Ubiquitin-AMC. 3. Read at excitation wavelengths 355nm and emission 460nm for 5 minutes.
- Ubiquitin-AMC (Enzo, Cat.No, BML-SE211)
- 96 Well Polystyrene Microplate, black (greiner bio-one, Cat.No, 655077)
- Fluorescent plate reader (PerkinElmer, VICTOR X3)
|Protein Description||Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques.|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|UniProt ID||P09936, V9HW74|
|Synonyms||HEL-117; HEL-S-53; NDGOA; PARK5; PGP 9.5; PGP9.5; PGP95; SPG79; Uch-L1|
|Summary||The protein encoded by this gene belongs to the peptidase C12 family. This enzyme is a thiol protease that hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. This gene is specifically expressed in the neurons and in cells of the diffuse neuroendocrine system. Mutations in this gene may be associated with Parkinson disease.[provided by RefSeq, Sep 2009]|
|Protein Families||Druggable Genome, Protease|
|Protein Pathways||Parkinson's disease|
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.