FKBP6 / FKBP36 (1-327, His-tag) Human Protein

SKU AR09470PU-N

FKBP6 / FKBP36 (1-327, His-tag) human recombinant protein, 50 µg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGGSALNQGV LEGDDAPGQS LYERLSQRML DISGDRGVLK DVIREGAGDL VAPDASVLVK YSGYLEHMDR PFDSNYFRKT PRLMKLGEDI TLWGMELGLL SMRRGELARF LFKPNYAYGT LGCPPLIPPN TTVLFEIELL DFLDCAESDK FCALSAEQQD QFPLQKVLKV AATEREFGNY LFRQNRFYDA KVRYKRALLL LRRRSAPPEE QHLVEAAKLP VLLNLSFTYL KLDRPTIALC YGEQALIIDQ KNAKALFRCG QACLLLTEYQ KARDFLVRAQ KEQPFNHDIN NELKKLASCY RDYVDKEKEM WHRMFAPCGD GSTAGES
• Tag: His-tag
• Predicted MW: 39.3 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Liquid purified protein; Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 40% glycerol, 1 mM EDTA, 0.2M NaCl
• Bioactivity: Specific: > 190 nmoles/min/mg, defined as the amount of enzyme that cleaves; 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin
• Preparation: Liquid purified protein
• Recommended Dilution: Protocol: Activity Assay; 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:; The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10 ul of recombinant FKBP6 protein with 1 ug in assay buffer.; 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25°C.
• Protein Description: Recombinant human FKBP6, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_001128683
• Locus ID: 8468
• UniProt ID: O75344
• Cytogenetics: 7q11.23
• Synonyms: FKBP-6, FKBP-36
• Summary: The protein encoded by this gene is a cis-trans peptidyl-prolyl isomerase that may function in immunoregulation and basic cellular processes involving protein folding and trafficking. This gene is located in a chromosomal region that is deleted in Williams-Beuren syndrome. Defects in this gene may cause male infertility. There are multiple pseudogenes for this gene located nearby on chromosome 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
• Protein Families: pH 8.0, Protocol: <strong>Activity Assay</strong><br />1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:<br />The final concentrations are 200 mM Tris-HCl