FKBP3 / FKBP25 (1-224) Human Protein

SKU AR09393PU-L

FKBP3 / FKBP25 (1-224) human recombinant protein, 0.5 mg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID
• Predicted MW: 25.1 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Liquid purified protein; Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
• Bioactivity: Specific: > 490 nmoles/min/mg, defined as the amount of enzyme that cleaves; 1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin
• Preparation: Liquid purified protein
• Recommended Dilution: Protocol: Activity Assay; 1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:; The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.; 2. Add 10 ul of recombinant FKBP3 protein with 1ug in assay buffer.; 3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.; 4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM); 5. Record the increase in A405 nm for 30 minutes at 25°C.
• Protein Description: Recombinant human FKBP3 was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_002004
• Locus ID: 2287
• UniProt ID: Q00688
• Cytogenetics: 14q21.2
• Synonyms: FKBP-3, FKBP-25
• Summary: The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. [provided by RefSeq, Sep 2008]
• Protein Families: pH 8.0, Protocol: <strong>Activity Assay</strong><br />1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:<br />The final concentrations are 200 mM Tris-HCl