Interleukin-15 / IL15 (49-162, His-tag)

CAT#: AR09050PU-N

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Recombinant human IL-15, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.


 Product Datasheet for 'AR09050PU-N'

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USD 400.00


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2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description Recombinant human IL-15, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Species Human
Expression Host E. coli
Tag His-tag
Predicted MW 13.9 kDa
Concentration 1.0 mg/ml
Purity > 95 % by SDS PAGE
Buffer PBS, pH 7.4, 0.2 mM PMSF, 10% glycerol
Bioactivity Measured in a cell proliferation assay using CTLL2 mouse cytotoxic
T cells. The ED50 for this effect is < 2.5 ng/ml.
Activity Assay
1.\tCell line: CTLL2 (mouse cytotoxic T cell)
2.\tMaintenance Condition: 10% FBS RPMI 1640 with hIL2
3.\tAssay Medium: 10% FBS RPMI 1640 without hIL2
4.\tCell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.\tStarvation : 24hr, Assay medium
6.\tIncubation Time: 24hr (after sample treatment)
7.\tConcentration Range: 0.039 ng/ml - 40 ng/ml
8.\tDetection Method : MTT assay
Endotoxin < 1.0 EU per 1 µg of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000576
Locus ID 3600
Cytogenetics 4q31.21
Synonyms IL-15
Summary The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other's activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Alternatively spliced transcript variants of this gene have been reported. [provided by RefSeq, Feb 2011]
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway

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68 Mouse Clones