Neurofilament L (68kDa)

CAT#: BA1012S

Neurofilament L (68kDa) bovine protein, 0.1 mg

Product Images

Lane 1 shows myosin (a), beta-galactosidase (b), phosphorylase B (c), BSA (d) and ovalbumin (e) as markers Lane 2 shows Cat.No. BA1014/BA1014S Neurofilament H (200 kD) Lane 3 shows Cat.No. BA1013/BA1013S Neurofilament M (160 kD) Lane 4 shows Cat.No. BA1012/BA1012S Neurofilament L (68kDa)

Specifications

Product Data

• Species: Bovine
• Protein Source: Spinal Cord
• Expression cDNA Clone or AA Sequence: sourced from bovine spinal cord
• Predicted MW: 68 kDa
• Concentration: lot specific
• Purity: >98% (determined by SDS gelelectrophoresis).
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized.
  Reconstitution: Restore with 80 µl distilled water (final volume 100 µl).
  Final solution: 10 mM Sodium Phosphate, pH 7.5, 2 mM DTT, 6M Urea, 10 mM Methylammonium Chloride, 1 mM EDTA
• Preparation: Lyophilized
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
• Protein Description: Standart Bovine Neurofilament 68 kD.
  Isoelectric Point: pI 5.0
• Storage: Store at 2-8°C (lyophilized) or at -20°C (reconstituted).
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF L, NF M and NF H. NF L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Neurofilaments constitute the main structural elements of neuronal axons and dendrites. Neurofilaments are composed of three major subunits referred to as the neurofilament triplet, with molecular weights of 68kD, 160kD and 200kD. Neurofilament subunits are present in neurons, neuronal processes, peripheral nerves and sympathetic ganglion cells.