GFAP Bovine Protein

SKU BA1011S

GFAP bovine protein, 0.1 mg

Product Data

• Species: Bovine
• Protein Source: Spinal Cord
• Predicted MW: 52 kDa
• Concentration: lot specific
• Purity: >98% (determined by SDS gelelectrophoresis).
• Presentation State: Purified
• Buffer: Presentation State: Purified; State: Lyophilized; Buffer System: Final Solution contains 10 mM Sodium Phosphate buffer pH 7.5, 6M Urea, 2 mM DTT, 1 mM EDTA, 10 mM Methylammonium Chloride
• Reconstitution Method: Restore with distilled water.; BA1011S: 80 µl (final volume 100 µl).; BA1011 : 200 µl (final volume 250 µl).
• Preparation: Lyophilized
• Recommended Dilution: Protein standard in 1D and 2D SDS gelelectrophoresis.; Immunoassays.; Immunization.; Protocol: Reconstitution to Filaments is performed by dissolving in 6 M urea buffer (see above) at concentrations of approx. 0.5 mg/ml. Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).; For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS).
• Protein Description: Bovine Glial Filament Protein (GFP)
• Note: Isoelectric Point: pI 5.4
• Storage: Prior to reconstitution store at 2-8°C.; Following reconstitution store the protein undiluted at -20°C.; Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_776490
• Locus ID: 281189
• Synonyms: Glial Fibrillary Acidic Protein
• Summary: glial fibrillary acidic protein
• Protein Families: 10 mM Tris-HCl, 2 mM dithiothreitol, e.g. Dulbeccos PBS)., pH 7.4).<br />For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline