PMF1 (NM_007221) Human Mass Spec Standard

CAT#: PH309547

PMF1 MS Standard C13 and N15-labeled recombinant protein (NP_009152)

Product Images

Coomassie blue staining of purified PMF1 protein (Cat# TP309547). The protein was produced from HEK293T cells transfected with PMF1 cDNA clone (Cat# RC209547) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC209547
• Predicted MW: 23.3 kDa
• Protein Sequence:
  Sequence Link

  >RC209547 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAEASSANLGSGCEEKRHEGSSSESVPPGTTISRVKLLDTMVDTFLQKLVAAGSYQRFTDCYKCFYQLQP
  AMTQRIYDKFIAQLQTSIREEISDIKEEGNLEAVLNALDKIVEEGKVRKEPAWRPSGIPEKDLHSVIAPY
  FLQQRDTLRRHVQKQEAENQQLADAVLAGRRQVEELQLQVQAQQQAWQALHREQRELVAVLREPE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_009152
• RefSeq Size: 1122
• RefSeq ORF: 615
• Locus ID: 11243
• UniProt ID: Q6P1K2
• Cytogenetics: 1q22
• Summary: Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis. May act as a cotranscription partner of NFE2L2 involved in regulation of polyamine-induced transcription of SSAT.[UniProtKB/Swiss-Prot Function]
• Protein Families: Stem cell - Pluripotency