POLR2L (NM_021128) Human Mass Spec Standard

CAT#: PH300649

POLR2L MS Standard C13 and N15-labeled recombinant protein (NP_066951)

Product Images

Coomassie blue staining of purified POLR2L protein (Cat# TP300649). The protein was produced from HEK293T cells transfected with POLR2L cDNA clone (Cat# RC200649) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC200649
• Predicted MW: 7.6 kDa
• Protein Sequence:
  Sequence Link

  >RC200649 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MIIPVRCFTCGKIVGNKWEAYLGLLQAEYTEGDALDALGLKRYCCRRMLLAHVDLIEKLLNYAPLEK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_066951
• RefSeq Size: 925
• RefSeq ORF: 201
• Synonyms: hRPB7.6; RBP10; RPABC5; RPB7.6; RPB10; RPB10beta
• Locus ID: 5441
• UniProt ID: P62875
• Cytogenetics: 11p15.5
• Summary: This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains four conserved cysteines characteristic of an atypical zinc-binding domain. Like its counterpart in yeast, this subunit may be shared by the other two DNA-directed RNA polymerases. [provided by RefSeq, Jul 2008]
• Protein Families: Transcription Factors
• Protein Pathways: Huntington's disease, Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase