DH10β Chemically Competent E. coli Cells

CAT#: CC100004

DH10β Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

Specifications

Product Data

• Genotype: F – mcrA ∆(mrr-hsdRMS-mcrBC) endA1 recA1 φ80dlacZ∆M15 ∆lacX74 araD139 ∆(ara, leu)7697 galU galK rpsL (StrR) nupG λ-
• Components: DH10β Chemically Competent Cells – 1 mL (5 x 200 μL)
  Positive Control DNA - pUC19 (50pg/μl, 25 μl )
  SOC medium – 20ml
• Features: Chemically Competent Accommodates small and larger plasmids RecA1 and endA1 markers for minimal recombination and enhanced quality of plasmid DNA
• Applications: lacZ marker for blue/white color screening
  Transformation of cloned DNA into bacterial cells
  Ideal for subcloning and generating cDNA libraries
• Storage: The Competent Cells should be stored at -80°C. pUC19 Control DNA should be stored at -20°C and SOC recovery medium should be stored at 4°C immediately upon arrival.
• Stability: The cells are stable for 6 months from the date of shipment.