Mouse Eef2k activation kit by CRISPRa

CAT#: GA201188

Eef2k CRISPRa kit - CRISPR gene activation of mouse eukaryotic elongation factor-2 kinase

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Eef2k
• Locus ID: 13631
• Kit Components:

  GA201188G1, Eef2k gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA201188G2, Eef2k gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA201188G3, Eef2k gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001251826, NM_001267710, NM_001267711, NM_007908
• UniProt ID: O08796
• Synonyms: C86191; eEF-2K
• Summary: Threonine kinase that regulates protein synthesis by controlling the rate of peptide chain elongation. Upon activation by a variety of upstream kinases including AMPK or TRPM7, phosphorylates the elongation factor EEF2 at a single site, renders it unable to bind ribosomes and thus inactive. In turn, the rate of protein synthesis is reduced.[UniProtKB/Swiss-Prot Function]