CACNA2D2 (NM_001174051) Human Untagged Clone

CAT#: SC329300

CACNA2D2 (untagged)-Human calcium channel voltage-dependent alpha 2/delta subunit 2 (CACNA2D2) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA2D2
• Synonyms: CACNA2D; CASVDD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC329300 representing NM_001174051.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGTGCCGGCTCGGACCTGCGGCGCCTCTCGGCCCGGCCCAGCGCGGACTGCGCGCCCCTGGCCC
  GGCTGCGGCCCCCACCCTGGCCCCGGCACCCGGCGCCCGACGTCCGGGCCCCCGCGCCCGCTGTGGCTG
  CTGCTGCCGCTTCTACCGCTGCTCGCCGCCCCCGGCGCCTCTGCCTACAGCTTCCCCCAGCAGCACACG
  ATGCAGCACTGGGCCCGGCGTCTGGAGCAGGAGGTCGACGGCGTGATGCGGATTTTTGGAGGCGTCCAG
  CAGCTCCGTGAGATTTACAAGGACAACCGGAACCTGTTCGAGGTACAGGAGAATGAGCCTCAGAAGTTG
  GTGGAGAAGGTGGCAGGGGACATTGAGAGCCTTCTGGACAGGAAGGTGCAGGCCCTGAAGAGACTGGCT
  GATGCTGCAGAGAACTTCCAGAAAGCACACCGCTGGCAGGACAACATCAAGGAGGAAGACATCGTGTAC
  TATGACGCCAAGGCTGACGCTGAGCTGGACGACCCTGAGAGTGAGGATGTGGAAAGGGGGTCTAAGGCC
  AGCACCCTAAGGCTGGACTTCATCGAGGACCCAAACTTCAAGAACAAGGTCAACTATTCATACGCGGCT
  GTACAGATCCCTACGGACATCTACAAAGGCTCCACTGTCATCCTCAATGAGCTCAACTGGACAGAGGCC
  CTGGAGAATGTGTTCATGGAAAACCGCAGACAAGACCCCACACTGCTGTGGCAGGTCTTCGGCAGCGCC
  ACAGGAGTCACTCGCTACTACCCGGCCACCCCGTGGCGAGCCCCCAAGAAGATCGACCTGTACGATGTC
  CGAAGGAGACCCTGGTATATCCAGGGGGCCTCGTCACCCAAAGACATGGTCATCATCGTGGATGTGAGT
  GGCAGTGTGAGCGGCCTGACCCTGAAGCTGATGAAGACATCTGTCTGCGAGATGCTGGACACGCTGTCT
  GATGATGACTATGTGAATGTGGCCTCGTTCAACGAGAAGGCACAGCCTGTGTCATGCTTCACACACCTG
  GTGCAGGCCAATGTGCGCAACAAGAAGGTGTTCAAGGAAGCTGTGCAGGGCATGGTGGCCAAGGGCACC
  ACAGGCTACAAGGCCGGCTTTGAGTATGCCTTTGACCAGCTGCAGAACTCCAACATCACTCGGGCCAAC
  TGCAACAAGATGATCATGATGTTCACGGATGGTGGTGAGGACCGCGTGCAGGACGTCTTTGAGAAGTAC
  AATTGGCCAAACCGGACGGTGCGCGTGTTTACTTTCTCCGTGGGGCAGCATAACTATGACGTCACACCG
  CTGCAGTGGATGGCCTGTGCCAACAAAGGCTACTATTTTGAGATCCCTTCCATCGGAGCCATCCGCATC
  AACACACAGGAATATCTAGATGTGTTGGGCAGGCCCATGGTGCTGGCAGGCAAGGAGGCCAAGCAGGTG
  CAGTGGACCAACGTGTATGAGGATGCACTGGGACTGGGGTTGGTGGTAACAGGGACCCTCCCTGTTTTC
  AACCTGACACAGGATGGCCCTGGGGAAAAGAAGAACCAGCTGATCCTGGGCGTGATGGGCATTGACGTG
  GCTCTGAATGACATCAAGAGGCTGACCCCCAACTACACGCTTGGAGCCAACGGCTATGTGTTTGCCATT
  GACCTGAACGGCTACGTGTTGCTGCACCCCAATCTCAAGCCCCAGACCACCAACTTCCGGGAGCCTGTG
  ACTCTGGACTTCCTGGATGCGGAGCTAGAGGATGAGAACAAGGAAGAGATCCGTCGGAGCATGATTGAT
  GGCAACAAGGGCCACAAGCAGATCAGAACGTTGGTCAAGTCCCTGGATGAGAGGTACATAGATGAGGTG
  ACACGGAACTACACCTGGGTGCCTATAAGGAGCACTAACTACAGCCTGGGGCTGGTGCTCCCACCCTAC
  AGCACCTTCTACCTCCAAGCCAATCTCAGTGACCAGATCCTGCAGGTCAAGTTGCCAATCAGCAAACTG
  AAGGATTTTGAGTTCCTGCTCCCCAGCAGCTTTGAGTCTGAAGGACACGTTTTCATTGCTCCCAGAGAG
  TACTGCAAGGACCTGAATGCCTCAGACAACAACACCGAGTTCCTGAAAAACTTTATTGAGCTCATGGAG
  AAAGTGACTCCAGACTCCAAGCAGTGCAACAACTTCCTTCTGCACAACCTGATCTTGGACACGGGCATC
  ACGCAGCAGCTGGTAGAGCGTGTGTGGAGGGACCAGGATCTCAACACGTACAGCCTACTGGCCGTGTTC
  GCTGCCACAGACGGTGGCATCACCCGAGTCTTCCCCAACAAGGCAGCTGAGGACTGGACAGAGAACCCT
  GAGCCCTTCAATGCCAGCTTCTACCGCCGCAGCCTGGATAACCACGGTTATGTCTTCAAGCCCCCACAC
  CAGGATGCCCTGTTAAGGCCGCTGGAGCTGGAGAATGACACTGTGGGCATCCTCGTCAGCACAGCTGTG
  GAGCTCAGCCTAGGCAGGCGCACACTGAGGCCAGCAGTGGTGGGCGTCAAGCTGGACCTAGAGGCTTGG
  GCTGAGAAGTTCAAGGTGCTAGCCAGCAACCGTACCCACCAAGACCAGCCTCAGAAGTGCGGCCCCAAC
  AGCCACTGTGAGATGGACTGCGAGGTTAACAATGAGGACTTACTCTGTGTCCTCATTGATGATGGAGGA
  TTCCTGGTGCTGTCAAACCAGAACCATCAGTGGGACCAGGTGGGCAGGTTCTTCAGTGAGGTGGATGCC
  AACCTGATGCTGGCACTCTACAATAACTCCTTCTACACCCGCAAGGAGTCCTATGACTATCAGGCAGCC
  TGTGCCCCTCAGCCCCCTGGCAACCTGGGTGCTGCACCCCGGGGTGTCTTTGTGCCCACCGTTGCAGAT
  TTCCTTAACCTGGCCTGGTGGACCTCTGCTGCCGCCTGGTCCCTGTTCCAGCAGCTTCTCTACGGCCTC
  ATCTACCACAGCTGGTTCCAAGCAGACCCCGCGGAGGCCGAGGGGAGCCCCGAGACGCGCGAGAGCAGC
  TGCGTCATGAAACAGACCCAGTACTACTTCGGCTCGGTAAACGCCTCCTACAACGCCATCATCGACTGC
  GGAAACTGCTCCAGGCTGTTCCACGCGCAGAGACTGACCAACACCAATCTTCTCTTTGTGGTGGCCGAG
  AAGCCGCTGTGCAGCCAGTGCGAGGCTGGCCGGCTGCTGCAGAAGGAGACGCACTCGGACGGCCCGGAG
  CAGTGTGAGCTAGTGCAGAGACCGCGATACCGGAGAGGCCCGCACATCTGCTTCGACTACAACGCGACA
  GAAGATACCTCAGACTGTGGCCGCGGGGCCTCCTTCCCGCCGTCGCTGGGCGTCCTGGTCTCCCTGCAA
  CTGCTGCTCCTCCTGGGCCTGCCGCCCCGGCCGCAGCCTCAAGTCCTCGTCCACGCCTCTCGCCGCCTC
  TGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001174051
• Insert Size: 3453 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001174051.2
• RefSeq Size: 5680 bp
• RefSeq ORF: 3453 bp
• Locus ID: 9254
• UniProt ID: Q9NY47
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome, Ion Channels: Other
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway
• MW: 129.8 kDa
• Gene Summary: Calcium channels mediate the entry of calcium ions into the cell upon membrane polarization. This gene encodes the alpha-2/delta subunit of the voltage-dependent calcium channel complex. The complex consists of the main channel-forming subunit alpha-1, and auxiliary subunits alpha-2/delta, beta, and gamma. The auxiliary subunits function in the assembly and membrane localization of the complex, and modulate calcium currents and channel activation/inactivation kinetics. The subunit encoded by this gene undergoes post-translational cleavage to yield the extracellular alpha2 peptide and a membrane-anchored delta polypeptide. This subunit is a receptor for the antiepileptic drug, gabapentin. Mutations in this gene are associated with early infantile epileptic encephalopathy. Single nucleotide polymorphisms in this gene are correlated with increased sensitivity to opioid drugs. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c, also known as Alpha2delta-2a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.