PGBD1 (NM_001184743) Human Untagged Clone

SKU SC329146

PGBD1 (untagged)-Human piggyBac transposable element derived 1 (PGBD1) transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: PGBD1
• Synonyms: dJ874C20.4; HUCEP-4; SCAND4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001184743, the custom clone sequence may differ by one or more nucleotides; ATGTATGAAGCTTTGCCAGGCCCTGCTCCTGAAAATGAAGATGGCCTTGTGAAAGTGAAG; GAGGAAGATCCCACCTGGGAGCAGGTGTGCAACTCACAGGAGGGCAGCTCCCACACTCAG; GAGATTTGCCGCCTGCGCTTTCGGCACTTCTGCTACCAGGAGGCTCACGGACCCCAGGAA; GCTCTGGCCCAACTCCGAGAACTTTGTCATCAATGGCTGAGACCGGAGATGCACACCAAG; GAACAGATAATGGAACTGCTGGTGCTGGAGCAGTTCCTGACCATCCTGCCCAAGGAGCTC; CAGCCCTGTGTGAAGACATATCCTCTGGAGAGTGGAGAGGAGGCAGTGACAGTGCTGGAG; AATCTAGAGACAGGAAGTGGAGACACAGGACAACAGGCCTCTGTCTATATTCAGGGACAG; GACATGCACCCAATGGTGGCAGAATATCAAGGAGTCTCTTTGGAGTGTCAGAGCCTCCAG; CTCCTGCCTGGGATAACCACCCTGAAGTGTGAACCTCCACAGCGTCCTCAAGGGAACCCC; CAAGAAGTGAGTGGGCCTGTTCCCCACGGATCAGCTCATCTCCAGGAAAAAAACCCCAGA; GACAAGGCTGTAGTGCCTGTGTTTAACCCAGTCAGGTCCCAGACATTGGTGAAGACTGAG; GAAGAAACAGCCCAGGCCGTTGCTGCAGAGAAGTGGTCACATCTGAGTCTGACTCGGAGG; AACCTCTGTGGGAACTCAGCTCAGGAGACAGTTATGAGCCTCAGTCCGATGACTGAAGAA; ATTGTAACTAAAGATAGATTGTTTAAAGCAAAGCAAGAAACTTCTGAAGAAATGGAACAA; AGTGGAGAAGCCTCAGGAAAGCCCAACAGAGAGTGTGCACCCCAGATTCCTTGTAGTACT; CCTATTGCTACTGAAAGGACAGTTGCACATTTGAACACTCTGAAGGACCGTCACCCAGGT; GATTTGTGGGCCCGCATGCACATCTCATCCCTGGAATATGCTGCAGGAGACATTACCCGA; AAAGGGAGAAAAAAAGACAAAGCTCGAGTGAGTGAACTGCTCCAAGGCCTCTCATTCTCT; GGTGACTCAGATGTGGAAAAAGATAATGAGCCTGAGATCCAGCCTGCTCAAAAGAAGTTA; AAGGTATCATGTTTCCCAGAAAAGAGTTGGACCAAAAGAGACATTAAACCCAATTTTCCA; AGCTGGTCAGCACTGGATTCTGGACTTTTGAATCTCAAGAGCGAAAAGTTGAACCCAGTA; GAGCTTTTTGAATTATTTTTTGATGATGAAACATTCAACTTAATTGTCAATGAAACCAAT; AATTATGCTTCTCAGAAAAATGTCAGCTTGGAAGTCACAGTTCAGGAAATGAGGTGTGTG; TTTGGTGTCTTACTTTTGAGTGGATTTATGAGGCATCCTAGAAGGGAAATGTATTGGGAA; GTCTCTGACACCGATCAGAACCTGGTTAGAGATGCAATCAGAAGGGACAGATTTGAATTG; ATTTTCTCAAACCTGCACTTTGCAGATAATGGCCACCTAGATCAAAAAGATAAGTTTACA; AAGTTGAGACCTCTCATAAAACAAATGAATAAAAATTTCCTCTTGTATGCTCCCCTGGAA; GAATACTATTGCTTTGATAAGTCAATGTGTGAATGCTTTGATAGTGACCAATTCCTGAAT; GGAAAGCCTATTAGAATTGGCTATAAAATTTGGTGTGGTACAACCACACAGGGTTATCTG; GTTTGGTTTGAACCCTATCAAGAAGAATCAACTATGAAGGTAGATGAGGATCCTGATCTT; GGGTTAGGTGGAAATCTAGTGATGAACTTCGCTGATGTTCTTTTAGAGAGAGGTCAGTAT; CCCTATCACCTGTGTTTTGATAGCTTCTTTACAAGTGTCAAATTGTTGTCAGCCTTGAAA; AAGAAGGGGGTGAGGGCAACAGGAACAATTCGTGAGAACAGGACCGAAAAATGTCCCCTT; ATGAATGTAGAACATATGAAAAAAATGAAGAGAGGGTATTTTGATTTCCGAATAGAAGAA; AACAATGAGATAATTTTGTGTCGTTGGTATGGGGATGGCATTATCAGTCTGTGCTCCAAT; GCTGTGGGCATAGAACCAGTCAATGAGGTAAGCTGTTGTGATGCTGATAACGAAGAAATC; CCTCAGATAAGTCAACCATCCATAGTAAAAGTGTATGATGAATGCAAGGAAGGTGTAGCT; AAAATGGATCAAATTATTTCGAAATACAGGGTGAGGATAAGAAGCAAGAAATGGTACTCA; ATTTTGGTGAGCTACATGATTGATGTAGCCATGAACAATGCATGGCAACTACACAGAGCC; TGTAACCCAGGTGCTTCTCTAGACCCCTTGGATTTTCGGAGATTTGTTGCACATTTCTAC; TTGGAACACAATGCTCATCTGTCAGATTAG;
• Restriction Sites: Please inquire
• ACCN: NM_001184743
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001184743.1, NP_001171672.1
• RefSeq Size: 3155 bp
• RefSeq ORF: 2430 bp
• Locus ID: 84547
• UniProt ID: Q96JS3
• Cytogenetics: 6p22.1
• Protein Families: Druggable Genome, Transcription Factors
• Summary: The piggyBac family of proteins, found in diverse animals, are transposases related to the transposase of the canonical piggyBac transposon from the moth, Trichoplusia ni. This family also includes genes in several genomes, including human, that appear to have been derived from the piggyBac transposons. This gene belongs to the subfamily of piggyBac transposable element derived (PGBD) genes. The PGBD proteins appear to be novel, with no obvious relationship to other transposases, or other known protein families. This gene product is specifically expressed in the brain, however, its exact function is not known. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, May 2010]; Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein.