SHANK2 (NM_133266) Human Untagged Clone

SKU SC328058

SHANK2 (untagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2) transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: SHANK2
• Synonyms: AUTS17; CORTBP1; CTTNBP1; ProSAP1; SHANK; SPANK-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_133266, the custom clone sequence may differ by one or more nucleotides; ATGATGATGAACGTCCCCGGCGGAGGAGCGGCCGCGGTGATGATGACGGGCTACAATAAT; GGTCGCTGTCCCCGGAATTCTCTCTACAGTGACTGCATTATTGAGGAGAAGACGGTGGTC; CTGCAGAAAAAAGACAATGAGGGCTTTGGATTCGTGCTTCGAGGGGCCAAAGCTGACACA; CCCATTGAAGAATTCACACCAACACCGGCTTTCCCAGCCCTACAGTACCTGGAGTCCGTG; GATGAAGGTGGGGTGGCGTGGCAAGCCGGACTAAGGACCGGGGACTTCTTGATTGAGGTT; AACAATGAGAATGTTGTCAAAGTCGGCCACAGGCAGGTGGTGAACATGATCCGGCAGGGA; GGGAATCACCTGGTCCTTAAGGTGGTCACGGTGACCAGGAATCTGGACCCCGACGACACC; GCCAGGAAGAAAGCTCCCCCGCCTCCAAAGCGGGCACCGACCACAGCCCTCACCCTGCGC; TCCAAGTCCATGACCTCGGAGCTGGAGGAGCTCGTGGATAAAGCCTCGGTCCGGAAGAAG; AAGGATAAACCCGAGGAGATAGTCCCGGCCTCCAAGCCCTCCCGCGCTGCTGAGAACATG; GCTGTGGAACCGAGGGTGGCGACCATCAAGCAGCGGCCCAGCAGCCGGTGCTTCCCGGCG; GGCTCAGACATGAACTCTGTGTACGAACGCCAAGGAATCGCCGTGATGACGCCCACTGTT; CCTGGGAGCCCAAAAGCCCCGTTTCTGGGCATCCCTCGAGGTACGATGCGAAGGCAGAAA; TCAATAGACAGCAGAATCTTTCTATCAGGAATAACAGAGGAAGAGCGGCAGTTTCTGGCT; CCTCCAATGCTGAAGTTCACCAGAAGCCTGTCCATGCCGGACACCTCTGAGGACATCCCC; CCTCCACCGCAGTCTGTGCCCCCGTCCCCACCACCACCTTCCCCAACCACTTACAACTGC; CCCAAGTCCCCAACTCCAAGAGTCTACGGGACGATTAAGCCTGCGTTCAATCAGAATTCT; GCCGCCAAGGTGTCCCCCGCCACCAGGTCCGACACCGTGGCCACCATGATGAGGGAGAAG; GGGATGTACTTCAGGAGAGAGCTGGACCGCTACTCCTTGGACTCTGAAGACCTCTACAGT; CGGAATGCCGGCCCGCAAGCCAACTTCCGCAACAAGAGAGGCCAGATGCCAGAAAACCCA; TACTCAGAGGTGGGGAAGATCGCCAGCAAAGCCGTCTACGTCCCCGCCAAGCCCGCCAGG; CGGAAGGGGATGCTGGTGAAGCAGTCCAACGTGGAGGACAGCCCCGAGAAGACGTGCTCC; ATCCCTATCCCGACCATCATCGTGAAGGAGCCGTCCACCAGCAGCAGCGGCAAGAGCAGC; CAGGGCAGCAGCATGGAGATCGACCCCCAGGCCCCGGAGCCACCGAGCCAGCTGCGGCCT; GACGAAAGCCTGACCGTCAGCAGCCCCTTTGCCGCCGCCATCGCCGGAGCCGTCCGCGAC; CGTGAGAAGCGGCTGGAAGCCAGGAGGAACTCCCCGGCCTTCCTCTCCACAGACCTGGGG; GATGAGGATGTGGGCCTGGGGCCACCCGCCCCCAGGACGCGGCCCTCCATGTTCCCCGAG; GAGGGGGATTTTGCTGACGAGGACAGCGCTGAGCAGCTGTCATCCCCCATGCCGAGTGCC; ACGCCCAGGGAGCCCGAAAACCATTTCGTGGGTGGCGCCGAGGCCAGTGCTCCGGGTGAG; GCTGGGAGGCCGCTGAATTCCACGTCCAAAGCCCAGGGGCCCGAGAGCAGCCCAGCAGTG; CCCTCCGCGAGCAGCGGCACAGCCGGCCCCGGGAATTATGTCCACCCACTCACAGGGCGG; CTGCTTGATCCCAGCTCCCCGCTGGCCCTGGCACTCTCCGCAAGGGACCGAGCCATGAAG; GAGTCTCAACAGGGACCCAAAGGGGAGGCCCCCAAGGCCGACCTCAACAAACCTCTTTAC; ATTGATACCAAAATGCGGCCCAGCCTGGATGCCGGCTTCCCTACGGTCACCAGGCAGAAC; ACCCGGGGACCCCTGAGGCGGCAGGAGACGGAGAACAAGTACGAGACCGACCTGGGCCGA; GACCGGAAAGGCGATGACAAGAAGAACATGCTGATCGACATCATGGACACGTCCCAGCAG; AAGTCGGCTGGCCTGCTGATGGTGCACACCGTGGACGCCACTAAGCTGGACAACGCCCTG; CAGGAAGAGGACGAGAAGGCAGAGGTGGAGATGAAGCCAGACAGCTCGCCGTCCGAGGTG; CCAGAAGGTGTTTCCGAAACCGAAGGTGCTTTACAGATCTCCGCTGCCCCCGAGCCCACC; ACCGTGCCCGGCAGAACCATCGTCGCGGTGGGCTCCATGGAAGAGGCGGTGATTTTGCCA; TTCCGCATCCCTCCTCCCCCTCTGGCATCCGTGGACTTGGATGAGGATTTTATTTTTACA; GAGCCATTGCCTCCTCCCCTGGAATTTGCAAATAGTTTTGATATCCCCGATGACCGGGCA; GCTTCTGTCCCGGCTCTCTCAGACTTAGTGAAGCAGAAGAAAAGCGACACCCCTCAGTCC; CCTTCGTTGAACTCCAGCCAACCAACCAACTCTGCAGACAGCAAGAAGCCAGCCAGTCTT; TCAAACTGTCTGCCTGCCTCATTCCTGCCACCCCCTGAAAGCTTTGACGCCGTCGCCGAC; TCTGGGATCGAGGAGGTGGACAGCCGGAGTAGCAGCGACCACCACCTCGAGACGACCAGC; ACTATCTCCACCGTGTCTAGCATCTCCACCCTGTCTTCCGAAGGTGGAGAGAATGTGGAC; ACCTGCACAGTCTATGCAGATGGGCAAGCATTTATGGTTGACAAACCCCCAGTACCTCCT; AAGCCAAAAATGAAGCCCATCATTCACAAAAGCAATGCACTTTATCAAGACGCGCTCGTG; GAAGAAGATGTAGATAGCTTTGTTATCCCCCCGCCCGCTCCCCCGCCCCCGCCGGGCAGT; GCCCAGCCTGGGATGGCCAAGGTTCTCCAGCCAAGGACCTCCAAGTTGTGGGGCGACGTC; ACAGAGATCAAAAGCCCGATTCTCTCAGGCCCAAAGGCAAACGTTATTAGTGAATTGAAC; TCTATCCTACAGCAAATGAACCGAGAGAAATTGGCAAAGCCGGGGGAAGGACTGGATTCA; CCAATGGGAGCCAAGTCCGCCAGCCTCGCTCCAAGAAGCCCGGAGATCATGAGCACCATC; TCAGGTACACGGAGCACGACGGTCACCTTCACTGTTCGCCCCGGCACCTCCCAGCCCATC; ACCCTGCAGAGCCGGCCCCCCGACTATGAAAGCAGGACCTCAGGAACAAGACGTGCCCCA; AGCCCTGTGGTCTCGCCAACAGAGATGAACAAAGAGACCCTGCCCGCCCCCCTGTCTGCT; GCCACCGCCTCTCCTTCTCCCGCTCTCTCAGATGTCTTTAGCCTTCCAAGCCAGCCCCCT; TCTGGGGATCTATTTGGCTTGAACCCAGCGGGACGCAGTAGGTCGCCATCCCCCTCGATA; CTGCAACAGCCAATCTCAAATAAGCCTTTTACAACTAAACCTGTCCACCTGTGGACTAAA; CCAGATGTGGCCGATTGGCTGGAAAGTCTAAACTTGGGTGAACATAAAGAGGCCTTCATG; GACAATGAGATCGATGGCAGTCACTTACCAAACCTGCAGAAGGAGGACCTCATCGATCTT; GGGGTAACTCGAGTCGGGCACAGAATGAACATAGAAAGGGCTTTGAAACAGCTGCTGGAC; AGA;
• Restriction Sites: Please inquire
• ACCN: NM_133266
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_133266.3, NP_573573.2
• RefSeq Size: 8850 bp
• RefSeq ORF: 3786 bp
• Locus ID: 22941
• UniProt ID: Q9UPX8
• Cytogenetics: 11q13.3-q13.4
• Summary: This gene encodes a protein that is a member of the Shank family of synaptic proteins that may function as molecular scaffolds in the postsynaptic density of excitatory synapses. Shank proteins contain multiple domains for protein-protein interaction, including ankyrin repeats, and an SH3 domain. This particular family member contains a PDZ domain, a consensus sequence for cortactin SH3 domain-binding peptides and a sterile alpha motif. The alternative splicing demonstrated in Shank genes has been suggested as a mechanism for regulating the molecular structure of Shank and the spectrum of Shank-interacting proteins in the postsynaptic densities of the adult and developing brain. Alterations in the encoded protein may be associated with susceptibility to autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]; Transcript Variant: This variant (2) differs in its 5' UTR, lacks a portion of the 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.