Metabotropic Glutamate Receptor 5 (GRM5) (NM_001143831) Human Untagged Clone

SKU SC326357

GRM5 (untagged)-Human glutamate receptor, metabotropic 5 (GRM5), transcript variant a

Product Data

• Type: Human Untagged Clone
• Target Symbol: Metabotropic Glutamate Receptor 5
• Synonyms: GPRC1E; mGlu5; MGLUR5; PPP1R86
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001143831 edited; CAGCCAAGGCCGCTACGAGAGCGCAGGAAGCCCTCGAGGAGCGGCTGCCAGGGGGCGCAC; GGCTCAGGGCGCGCACCTGGTTTAGAAGATCATGACCACATGGATCATCTAACTAAATGG; TACATGGGGACAAAATGGTCCTTTAGAAAATACATCTGAATTGCTGGCTAATTTCTTGAT; TTGCGACTCAACGTAGGACATCGCTTGTTCGTAGCTATCAGAACCCTCCTGAATTTTCCC; CACCATGCTATCTTTATTGGCTTGAACTCCTTTCCTAAAATGGTCCTTCTGTTGATCCTG; TCAGTCTTACTTTTGAAAGAAGATGTCCGTGGGAGTGCACAGTCCAGTGAGAGGAGGGTG; GTGGCTCACATGCCGGGTGACATCATTATTGGAGCTCTCTTTTCTGTTCATCACCAGCCT; ACTGTGGACAAAGTTCATGAGAGGAAGTGTGGGGCGGTCCGTGAACAGTATGGCATTCAG; AGAGTGGAGGCCATGCTGCATACCCTGGAAAGGATCAATTCAGACCCCACACTCTTGCCC; AACATCACACTGGGCTGTGAGATAAGGGACTCCTGCTGGCATTCGGCTGTGGCCCTAGAG; CAGAGCATTGAGTTCATAAGAGATTCCCTCATTTCTTCAGAAGAGGAAGAAGGCTTGGTA; CGCTGTGTGGATGGCTCCTCCTCTTCCTTCCGCTCCAAGAAGCCCATAGTAGGGGTCATT; GGGCCTGGCTCCAGTTCTGTAGCCATTCAGGTCCAGAATTTGCTCCAGCTTTTCAACATA; CCTCAGATTGCTTACTCAGCAACCAGCATGGATCTGAGTGACAAGACTCTGTTCAAATAT; TTCATGAGGGTTGTGCCTTCAGATGCTCAGCAGGCAAGGGCCATGGTGGACATAGTGAAG; AGGTACAACTGGACCTATGTATCAGCCGTGCACACAGAAGGCAACTATGGAGAAAGTGGG; ATGGAAGCCTTCAAAGATATGTCAGCGAAGGAAGGGATTTGCATCGCCCACTCTTACAAA; ATCTACAGTAATGCAGGGGAGCAGAGCTTTGATAAGCTGCTGAAGAAGCTCACAAGTCAC; TTGCCCAAGGCCCGGGTGGTGGCCTGCTTCTGTGAGGGCATGACGGTGAGAGGTCTGCTG; ATGGCCATGAGGCGCCTGGGTCTAGCGGGAGAATTTCTGCTTCTGGGCAGTGATGGCTGG; GCTGACAGGTATGATGTGACAGATGGATATCAGCGAGAAGCTGTTGGTGGCATCACAATC; AAGCTCCAATCTCCCGATGTCAAGTGGTTTGATGATTATTATCTGAAGCTCCGGCCAGAA; ACAAACCACCGAAACCCTTGGTTTCAAGAATTTTGGCAGCATCGTTTTCAGTGCCGACTG; GAAGGGTTTCCACAGGAGAACAGCAAATACAACAAGACTTGCAATAGTTCTCTGACTCTG; AAAACACATCATGTTCAGGATTCCAAAATGGGATTTGTGATCAACGCCATCTATTCGATG; GCCTATGGGCTCCACAACATGCAGATGTCCCTCTGCCCAGGCTATGCAGGACTCTGTGAT; GCCATGAAGCCAATTGATGGACGGAAACTTTTGGAGTCCCTGATGAAAACCAATTTTACT; GGGGTTTCTGGAGATACGATCCTATTCGATGAGAATGGAGACTCTCCAGGAAGGTATGAA; ATAATGAATTTCAAGGAAATGGGAAAAGATTACTTTGATTATATCAACGTTGGAAGTTGG; GACAATGGAGAATTAAAAATGGATGATGATGAAGTATGGTCCAAGAAAAGCAACATCATC; AGATCTGTGTGCAGTGAACCATGTGAGAAAGGCCAGATCAAGGTGATCCGAAAGGGAGAA; GTCAGCTGTTGTTGGACCTGTACACCTTGTAAGGAGAATGAGTATGTCTTTGATGAGTAC; ACATGCAAGGCATGCCAACTGGGGTCTTGGCCCACTGATGATCTCACAGGTTGTGACTTG; ATCCCAGTACAGTATCTTCGATGGGGTGACCCTGAACCCATTGCAGCTGTGGTGTTTGCC; TGCCTTGGCCTCCTGGCCACCCTGTTTGTTACTGTAGTCTTCATCATTTACCGTGATACA; CCAGTAGTCAAGTCCTCAAGCAGGGAACTCTGCTACATTATCCTTGCTGGCATCTGCCTG; GGCTACTTATGTACCTTCTGCCTCATTGCGAAGCCCAAACAGATTTACTGCTACCTTCAG; AGAATTGGCATTGGTCTCTCCCCAGCCATGAGCTACTCAGCCCTTGTAACAAAGACCAAC; CGTATTGCAAGGATCCTGGCTGGCAGCAAGAAGAAGATCTGTACCAAAAAGCCCAGATTC; ATGAGTGCCTGTGCCCAGCTAGTGATTGCTTTCATTCTCATATGCATCCAGTTGGGCATC; ATCGTTGCCCTCTTTATAATGGAGCCTCCTGACATAATGCATGACTACCCAAGCATTCGA; GAAGTCTACCTGATCTGTAACACCACCAACCTAGGAGTTGTCACTCCACTTGGATACAAT; GGATTGTTGATTTTGAGCTGCACCTTCTATGCGTTCAAGACCAGAAATGTTCCAGCTAAC; TTCAACGAGGCCAAGTATATCGCCTTCACAATGTACACGACCTGCATTATATGGCTAGCT; TTTGTGCCAATCTACTTTGGCAGCAACTACAAAATCATCACCATGTGTTTCTCGGTCAGC; CTCAGTGCCACAGTGGCCCTAGGCTGCATGTTTGTGCCGAAGGTGTACATCATCCTGGCC; AAACCAGAGAGAAACGTGCGCAGCGCCTTCACCACATCTACCGTGGTGCGCATGCATGTA; GGGGATGGCAAGTCATCCTCCGCAGCCAGCAGATCCAGCAGCCTAGTCAACCTGTGGAAG; AGAAGGGGCTCCTCTGGGGAAACCTTAAGGTACAAAGACAGGAGACTGGCCCAGCACAAG; TCGGAAATAGAGTGTTTCACCCCCAAAGGGAGTATGGGGAATGGTGGGAGAGCAACAATG; AGCAGTTCCAATGGAAAATCCGTCACGTGGGCCCAGAATGAGAAGAGCAGCCGGGGGCAG; CACCTGTGGCAGCGCCTGTCCATCCACATCAACAAGAAAGAAAACCCCAACCAAACGGCC; GTCATCAAGCCCTTCCCCAAGAGCACGGAGAGCCGTGGCCTGGGCGCTGGCGCTGGCGCA; GGCGGGAGCGCTGGGGGCGTGGGGGCCACGGGCGGTGCGGGCTGCGCAGGCGCCGGCCCA; GGCGGGCCCGAGTCCCCAGACGCCGGCCCCAAGGCGCTGTATGATGTGGCCGAGGCTGAG; GAGCACTTCCCGGCGCCCGCGCGGCCGCGCTCACCGTCGCCCATCAGCACGCTGAGCCAC; CGCGCGGGCTCGGCCAGCCGCACGGACGACGATGTGCCGTCGCTGCACTCGGAGCCTGTG; GCGCGCAGCAGCTCCTCGCAGGGCTCCCTCATGGAGCAGATCAGCAGTGTGGTCACCCGC; TTCACGGCCAACATCAGCGAGCTCAACTCCATGATGCTGTCCACCGCGGCCCCCAGCCCC; GGCGTCGGCGCCCCGCTCTGCTCGTCCTACCTGATCCCCAAAGAGATCCAGTTGCCCACG; ACCATGACGACCTTTGCCGAAATCCAGCCTCTGCCGGCCATCGAAGTCACGGGCGGCGCG; CAGCCCGCGGCAGGGGCGCAGGCGGCTGGGGACGCGGCCCGGGAGAGCCCCGCGGCCGGT; CCCGAGGCTGCGGCCGCCAAGCCAGACCTGGAGGAGCTGGTGGCTCTCACCCCGCCGTCC; CCCTTCAGAGACTCGGTGGACTCGGGGAGCACAACCCCCAACTCGCCAGTGTCCGAGTCG; GCCCTCTGTATCCCGTCGTCTCCCAAATATGACACTCTTATCATAAGAGATTACACTCAG; AGCTCCTCGTCGTTGTGAATGTCCCTGGAAAGCACGCCGGCCTGCGCGTGCGGAGCGGAG; CCCCCCGTGTTCACACACACACAATGGCAAGCATAGTCGCCTGGTTACGGCCCAGGGGGA; AGATGCCAAGGGCACCCCTTAATGGAAACACGAGATCAGTAGTGCTATCTCATGACAACC; GACGAAGAAACCGACGACAAATCTTTTGGCAGATTTTCTTCTAGTGGCCTTAGAAAACAT; GGGCTTTTAAGAAACACGGCTGATATCTTTGAGGGCTGACAAGGCGTCTCTTCAAACAGT; TCCATACCAAGTGCTTTGCTCTAGGGAAGCAGTGCGTGTGAAACAGCGTAACGGAGGGTG; AAGAGCATAGTTAATAAGCAACTGTAAAAAGTTTTATTTGTTTACTTTAATTCTTTTCCC; AGAAGAGTCTTTGATTCACCAAACATGAATGTACATTTTCTAACAAACTCAAAATCTGGG; ACCAAAACATCAACTTTTTTCTTTCTTTTTTCTTTCTTTTTGTTTTTTCTTTCCTGTAAA; GACCTTGAAAAGCAGTAACTTGGGTCCAGTATTTACGGAGGCGTTGTGAATGTGTCCCAT; GCATAACACACTACTGGATAGTGAGTGCTGCGCTAATGTACTACGTAGGGCTTCTACCAG; AGATTTTCCTCTCCAATTGGGTTGTGAAATACTCTTCCAAAAGCCTGCATCGGGGATTCC; ACCTACTTATTTCAGATTCACCTCCATTAACCAAGAAAACCAGTGGAAGATTTCTTGACT; ATTTCACCATGTTGCCAATCAATACTGGAGTAGCAAAAAAAATATTTTCTGGAATACTGT; TTTGTAATTCCCTCACTGGGGTGCATTGTAGCTGGAAATTCTCTTTATAAAAATCATTCT; TGAGCTCCAGCCTGGCTATCTCTTTCAAGAAACATGGCCACTCTTTAGGAATGCTGTTGC; GTTTGCATTGCCAACTAAAATATTAAAATATGCATTGGGGCTTCTTCATTCCTTTATTTT; GAGAACCTGATGCACAAAGAGCTCCTTTGTTCTTTTCGAGTCCCACCACTGGAAGAGTGG; TCCATAGACCCCATGAAGACATTGTCATGATTTGAGAGACTGTTGTTGAAAGGATTAACA; CAATCTTAATACACTGAAAATTTTAACTGTGTCAAGTCAGCTTAGTGGAGATTTAGCTAT; GCCAGTGAGCAGTGATTTTAACTATTCTTGGCTGCTTAAACAGGGCAGCTATGAACTATG; ACAAATGTAGATTTTTCAAAGCAATACAAAATACTAAAAAAGAGGAACCTTAATGAATAT; TAACCACACAGTCTTTCTTAGCCATTCCAAAAAGAGGCAAAGCAATTCTTATTTTCTTTT; TTAAAATAATGATTAATATGATTTTGTGCACTTCATACTGTCACTTTTTAAAACTACAGA; AAAGAGATTTAGAGTATAACAGAAACAAGTGTGCTTTGATAGTCTCAAATAGGTAGAATT; CATAGTTCAAGACCTGAATCCACTGTCATCTCTTTCTTCCTCCCATTGCAGCTATCCTCA; GGTACCAAATGTTTTGATTTTTAAATAAGGATAGTAATAAATGGAGGAGGTGTCCTATAA; ATTTAAAGTTCAGTTGACCCAGCCTTATACTTAAGATAGCCTTATGAAAAATATGTGCTG; TGAGGCAGAAGTATATTTTGGCAGAGAGAATAATAAATAAAACTTTTTCTTTTAGCTCAA; TATCCTTACTTTGGTAAGTATTTTTTTTTATTTCACATCTACTTAACAGAAAATAAACTG; AGAAATAGAAGTCAGTCCATTGGCATAATTTATCATTCTTCACTTTAAAAAATTCTAATA; AATATTCTGCTTGAGTTTTCTTTTCTGCTATTTGTTCTTACTTGCAACTTTAAGTCAAAC; CTCCCAATACAAAACATTAAAAGCTAACATTAATGTACTAAAGTATTAATTTAAAAGAAA; TCGAACCTCCCATGCTAGATTTGAAAATAACATCATCACAGCACCCTGATCCCAAATATT; ACACCGAGGCTTTTAAAATGTAAGTGAAATCTAGCTAAGTTTCATGGTTTCATTAAAAGC; AAATGTCTGCCTCTATCTGAAAAACAAATGGAAATCTTTTGAGGTGTTAATACCCTTTGG; ATCCTCATCAAAAGGATGGCATTCACCTGAGGATTCCTATCTTGACTTCTTAGGTATTAA; AAACCTTTCTTGATATGCTCTACATTTTAAAATTTGTTTTATAAAATCCTTATGTTGATT; TTCATTTTATTCTCAAGTACAATACGTTTCACTCTAGACCAGTTGAAGAACATGTTTAAA; CTTTGTTCATGGTCAAATTCATTTTCTATTTTTTTAGTAACATATCTCTTAAAAAGCACA; CTACCTTATAAAAAACTTCATCAGAAATTAAATTTAATGCAAGTAAATTGCCATCTGATA; CTTCCACATGCTATCATAATCAACTGTAATAATAAAAATGATTTATCCAATTAGAAAAGG; ACAAGATATATTTTTCTCTGTATTTCTATAACTTTTGCCACTCCATTGAATACATTGTAT; GTTGGACATAAGATTATTAGTAATGCATTCTTGAGATCTTTTATTTTGGAATGATGCTAA; CTCTGTCTCTTTGCCAATTCTAATACCAGGTTCCAAGTAATAACTCTACAGTACAAAGAG; AACTGAATATTCATTCTAGGGCTATAGGATATGAACTTCACAATTCATTTGGGTACATTC; TCATTGAATTTCCTTCAAAACAATCTGTTCCTGGTGCCCAGTGATAATTCAGTCGGGACC; AGCATGACTAAAAGGAAGGGGATATGCTAAGGCTCAGCAAAGTGACCCTAAATGAGAGAT; ATGTCCCAGGATGGAAAGAAGAAGACGTGGTTTAACCAAGTTATACTGACTAATCTAAGC; AGTCCACTCATCCTTCCATTTTGGGAAAGGAGTGGGGGCAGCCTAAGAAGAACATATCTG; GATTGGGAAGAACCGTCTTTCTGGGCTAGGGATGGGGAACAGAAAGGGAGTATGGAAAGA; AAAATTATAAGAGATTTGACTGAAGCAAGGAAAAAAAGCAAATCCCCAAACGTGCTAATC; CTTGAAAGTAACTATCTTTCCCAAACTACTGCTGTTACCAGCAAGTGATCAGGAAGACTA; GGAGCTATTTCTGACTGTAAATGAATTGTATAATAGCTCTGCTGCAGTTCTGTGACTTCC; AAGCCAGGAATTAAATGCTCTTTTTAAGAATAACAAAAAACAAAAGCATTTCCTATGCTA; GTCTCCCAGTAAAATGTACATGTTTTGGAGACTTCAAAGGTATTATGTGAGTTCACATTT; AGCAACAGCTTATTAATAACCCTCAAGCTGTCAGAATCTCTATAGTTACCATTTACAATT; TTATACTGTGAAAAAATACAGATCAGTGAAAGCATAAAGACAAGTCAGAATTCACTTTGA; AGAGGGTCTGAGGCCTGGGAGAGTCTCTACTGTCTATTGAAGAATGAGGCATGTATAAAA; TAGTTGGTTGAATTTCACTGATCTTCCCAATGTGAACAAATATACTATGTATATTGTGTG; TATTTCTAGAAATCAATGGCAGCTGCTGATGGTGTTGTAATTAGAAATCTATATAGATTA; TAGATGTTTTAGAAAGATGGTGCCAATCCTAAAAGATTTGTGTGGGCTAAAAGTGCTTGT; ACTTACTTTTTTCTGCACTTATAACTGATTTGGTTTTAAAATTGTGTGCGTGTATCTGTT; CTTTCTCTGTTGTGGCAGCTTGTACTATTAAAATAATAGAGAATGTTAAATTATTTTGAT; GTGAACTGCAAATGATTTTTTTTCATAAAGTTTAACATTTTTATCAGCATTGTTTTGCTT; TGTACTTGTATAAATATGTTTTATTTTAGCACTTCAAAATATACTTGCCTGTTTCTCAGT; TGTCTAAATCATGTTGTACTTGGTGTTTGTGAAGCCAGTTACTTTTCAAAAAAATTAAAA; AACCTATAATATGATTTAAAAAAAAAAAAAAAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_001143831
• Insert Size: 3639 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001143831.1.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001143831.1, NP_001137303.1
• RefSeq Size: 8062 bp
• RefSeq ORF: 3639 bp
• Locus ID: 2915
• UniProt ID: P41594
• Cytogenetics: 11q14.2-q14.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Calcium signaling pathway, Gap junction, Huntington's disease, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction
• Summary: This gene encodes a member of the G-protein coupled receptor 3 protein family. The encoded protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]; Transcript Variant: This variant (a) encodes the longer isoform (a, also known as mGluR5b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.