POLR1B (NM_001137604) Human Untagged Clone

SKU SC326333

POLR1B (untagged)-Human polymerase (RNA) I polypeptide B, 128kDa (POLR1B), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: POLR1B
• Synonyms: A135; RPA2; RPA135; Rpo1-2; TCS4
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001137604, the custom clone sequence may differ by one or more nucleotides; ATGGATCCTGGCAGCCGGTGGCGGAACCTGCCCAGCGGGCCTAGCCTAAAGCACTTGACT; GACCCCTCTTATGGAATCCCGCGGGAACAGCAAAAGGCAGCGTTGCAGGAGCTGACGCGG; GCGCACGTGGAGTCCTTCAACTACGCTGTGCACGAGGGTCTCGGCCTCGCGGTGCAGGCT; GATATCAACTGGGCAGTGAATGGAATCTCAAAAGGAATCATTAAGCAGTTTCTTGGCTAT; GTTCCCATCATGGTGAAATCCAAGCTTTGCAACTTACGTAACCTTCCCCCACAAGCCCTC; ATTGAGCACCATGAGGAGGCAGAGGAAATGGGGGGCTATTTTATAATCAATGGCATTGAA; AAAGTCATCCGAATGTTGATTATGCCTCGGAGAAATTTTCCCATTGCAATGATAAGACCA; AAATGGAAAACCAGAGGGCCTGGTTATACTCAGTATGGAGTTTCAATGCACTGTGTGAGG; GAAGAACATTCCGCTGTCAATATGAACCTCCACTACTTGGAAAATGGCACTGTTATGTTG; AACTTTATTTACCGAAAAGAACTGTTCTTTCTTCCTTTGGGATTTGCACTTAAGGCACTT; GTCAGCTTTTCTGATTATCAGATCTTTCAGGAGCTCATCAAAGGAAAAGAGGATGATTCT; TTCCTTAGGAACTCTGTTTCTCAGATGTTAAGGATTGTAATGGAAGAGGGTTGTTCGACA; CAAAAACAGGTCCTTAACTACCTAGGTGAATGCTTCAGAGTAAAACTCAATGTTCCTGAC; TGGTACCCAAATGAGCAAGCTGCGGAGTTCCTGTTTAACCAGTGCATCTGTATCCACTTG; AAATCCAATACTGAAAAGTTTTATATGCTTTGTCTCATGACGCGAAAGCTCTTTGCTTTA; GCCAAAGGAGAGTGCATGGAGGACAATCCTGATAGTTTGGTGAACCAGGAAGTCCTCACA; CCGGGTCAGCTCTTCCTTATGTTCCTGAAGGAAAAACTGGAAGGTTGGTTAGTGTCTATT; AAAATAGCTTTTGATAAGAAGGCTCAGAAGACCAGTGTTTCCATGAACACTGACAATTTG; ATGAGGATTTTTACAATGGGCATAGACCTTACAAAACCATTTGAATACCTTTTTGCTACT; GGGAATCTGCGTTCTAAAACAGGTCTTGGCCTCCTACAAGATTCTGGACTTTGTGTTGTG; GCTGACAAGCTGAACTTCATACGCTACCTCTCCCATTTCCGCTGCGTGCACAGAGGGGCT; GATTTTGCCAAGATGAGGACCACCACAGTACGCAGGCTGCTGCCAGAGTCCTGGGGCTTC; CTTTGTCCCGTGCATACCCCAGACGGGGAGCCCTGTGGCCTGATGAACCACCTAACTGCC; GTATGTGAGGTTGTCACACAGTTTGTGTATACGGCATCTATTCCAGCTTTACTGTGCAAC; TTGGGGGTCACTCCCATTGATGGAGCTCCCCACCGATCATACAGTGAGTGCTACCCTGTC; CTGCTGGACGGTGTCATGGTTGGCTGGGTGGATAAGGATCTTGCTCCAGGCATCGCAGAT; TCTCTTCGTCATTTTAAGGTGTTGAGAGAGAAAAGAATTCCTCCCTGGATGGAAGTGGTC; CTTATACCCATGACAGGAAAACCAAGTCTGTACCCAGGATTGTTCCTTTTTACCACTCCT; TGTAGACTGGTACGGCCTGTGCAGAACTTAGCATTGGGCAAAGAAGAGCTAATTGGAACT; ATGGAACAGATCTTCATGAATGTCGCTATCTTTGAGGATGAAGTTTTTGCTGGAGTTACC; ACACACCAGGAACTCTTTCCACACAGCCTGCTGAGTGTGATTGCCAACTTCATCCCTTTC; TCTGATCACAACCAGAGTCCACGGAACATGTACCAATGCCAGATGGGTAAGCAAACTATG; GGCTTTCCACTTCTCACTTATCAAGACCGATCGGATAACAAACTGTATCGTCTTCAGACT; CCTCAGAGTCCCTTGGTGAGACCCTCCATGTATGATTATTATGACATGGATAACTATCCA; ATTGGGACCAATGCCATCGTTGCTGTGATTTCTTACACTGGCTATGATATGGAAGATGCC; ATGATTGTGAATAAGGCCTCTTGGGAACGAGGCTTTGCCCATGGAAGTGTCTACAAGTCT; GAGTTCATAGACCTCTCTGAAAAAATTAAACAAGGAGATAGTAGCCTGGTGTTTGGCATC; AAACCTGGTGACCCACGCGTTCTGCAGAAGTTAGATGACGATGGATTGCCGTTTATAGGA; GCAAAACTGCAGTACGGAGATCCGTATTACAGCTACCTCAACCTCAACACCGGGGAAAGT; TTTGTGATGTACTATAAGAGTAAAGAAAATTGTGTTGTGGATAACATCAAAGTGTGCAGT; AATGACACTGGGAGTGGAAAATTCAAGTGTGTTTGCATCACTATGAGAGTGCCTCGGAAC; CCAACTATCGGAGATAAATTTGCCAGTCGCCATGGGCAGAAGGGCATTTTAAGCAGATTG; TGGCCGGCTGAGGACATGCCTTTTACTGAGAGTGGGATGGTCCCAGACATTCTGTTCAAT; CCCCATGGTTTTCCATCCCGCATGACCATTGGGATGTTAATTGAGAGTATGGCCGGGAAG; TCTGCAGCTTTGCATGGTCTCTGCCATGATGCTACACCCTTCATCTTCTCAGAGGAGAAC; TCGGCCTTAGAATACTTTGGTGAGATGTTAAAGGCTGCTGGCTACAATTTCTATGGCACC; GAGAGGTTATATAGTGGCATCAGTGGGCTAGAACTGGAAGCAGACATCTTCATAGGAGTG; GTTTATTATCAGCGCTTACGCCATATGGTCTCAGACAAATTTCAAGTAAGGACAACTGGA; GCCCGAGACAGAGTCACCAACCAGCCTATTGGGGGAAGAAATGTCCAGGGTGGAATCCGT; TTTGGGGAGATGGAACGGGATGCGCTTTTAGCTCATGGTACATCTTTTCTCCTTCATGAC; CGCCTCTTCAACTGCTCAGATCGGTCGGTAGCCCATGTGTGTGTGAAGTGTGGCAGTTTA; CTCTCTCCACTGTTGGAGAAGCCACCCCCTTCTTGGTCTGCCATGCGCAACAGAAAATAC; AACTGTACTCTGTGTAGTCGCAGTGACACTATCGATACTGTTTCTGTGCCTTATGTTTTT; CGGTATTTTGTAGCTGAACTGGCAGCTATGAACATCAAAGTGAAACTGGATGTTGTT;
• Restriction Sites: Please inquire
• ACCN: NM_001137604
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001137604.1, NP_001131076.1
• RefSeq Size: 5259 bp
• RefSeq ORF: 3240 bp
• Locus ID: 84172
• UniProt ID: Q9H9Y6
• Cytogenetics: 2q14.1
• Protein Families: Transcription Factors
• Protein Pathways: Metabolic pathways, Purine metabolism, Pyrimidine metabolism, RNA polymerase
• Summary: Eukaryotic RNA polymerase I (pol I) is responsible for the transcription of ribosomal RNA (rRNA) genes and production of rRNA, the primary component of ribosomes. Pol I is a multisubunit enzyme composed of 6 to 14 polypeptides, depending on the species. Most of the mass of the pol I complex derives from the 2 largest subunits, Rpa1 and Rpa2 in yeast. POLR1B is homologous to Rpa2 (Seither and Grummt, 1996 [PubMed 8921381]).[supplied by OMIM, Mar 2008]; Transcript Variant: This variant (2) lacks an alternate in-frame exon in the 5' coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.