RPA70 (RPA1) (NM_002945) Human Untagged Clone

CAT#: SC324660

RPA1 (untagged)-Human replication protein A1, 70kDa (RPA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPA70
• Synonyms: HSSB; MST075; REPA1; RF-A; RP-A; RPA70
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002945.2 GGAGCTGTTGCGGGGTCCGCGGGGAAGTCTTGGCGGTGGAGCCATGGTCGGCCAACTGAG
  CGAGGGGGCCATTGCGGCCATCATGCAGAAGGGGGATACAAACATAAAGCCCATCCTCCA
  AGTCATCAACATCCGTCCCATTACTACGGGGAATAGTCCGCCGCGTTATCGACTGCTCAT
  GAGTGATGGATTGAACACTCTATCCTCTTTCATGTTGGCGACACAGTTGAACCCTCTCGT
  GGAGGAAGAACAATTGTCCAGCAACTGTGTATGCCAGATTCACAGATTTATTGTGAACAC
  TCTGAAAGACGGAAGGAGAGTAGTTATCTTGATGGAATTAGAAGTTTTGAAGTCAGCTGA
  AGCAGTTGGAGTGAAGATTGGCAATCCAGTGCCCTATAATGAAGGACTCGGGCAGCCGCA
  AGTAGCTCCTCCAGCGCCAGCAGCCAGCCCAGCAGCAAGCAGCAGGCCCCAGCCGCAGAA
  TGGAAGCTCGGGAATGGGTTCTACTGTTTCTAAGGCTTATGGTGCTTCAAAGACATTTGG
  AAAAGCTGCAGGTCCCAGCCTGTCACACACTTCTGGGGGAACACAGTCCAAAGTGGTGCC
  CATTGCCAGCCTCACTCCTTACCAGTCCAAGTGGACCATTTGTGCTCGTGTTACCAACAA
  AAGTCAGATCCGTACCTGGAGCAACTCCCGAGGGGAAGGGAAGCTTTTCTCCCTAGAACT
  GGTTGACGAAAGTGGTGAAATCCGAGCTACAGCTTTCAATGAGCAAGTGGACAAGTTCTT
  TCCTCTTATTGAAGTGAACAAGGTGTATTATTTCTCGAAAGGCACCCTGAAGATTGCTAA
  CAAGCAGTTCACAGCTGTTAAAAATGACTACGAGATGACCTTCAATAACGAGACTTCCGT
  CATGCCCTGTGAGGACGACCATCATTTACCTACGGTTCAGTTTGATTTCACGGGGATTGA
  TGACCTCGAGAACAAGTCGAAAGACTCACTTGTAGACATCATCGGGATCTGCAAGAGCTA
  TGAAGACGCCACTAAAATCACAGTGAGGTCTAACAACAGAGAAGTTGCCAAGAGGAATAT
  CTACTTGATGGACACATCTGGGAAGGTGGTGACTGCTACACTGTGGGGGGAAGATGCTGA
  TAAATTTGATGGTTCTAGACAGCCCGTGTTGGCTATCAAAGGAGCCCGAGTCTCTGATTT
  CGGTGGACGGAGCCTCTCCGTGCTGTCTTCAAGCACTATCATTGCGAATCCTGACATCCC
  AGAGGCCTATAAGCTTCGTGGATGGTTTGACGCAGAAGGACAAGCCTTAGATGGTGTTTC
  CATCTCTGATCTAAAGAGCGGCGGAGTCGGAGGGAGTAACACCAACTGGAAAACCTTGTA
  TGAGGTCAAATCCGAGAACCTGGGCCAAGGCGACAAGCCGGACTACTTTAGTTCTGTGGC
  CACAGTGGTGTATCTTCGCAAAGAGAACTGCATGTACCAAGCCTGCCCGACTCAGGACTG
  CAATAAGAAAGTGATTGATCAACAGAATGGATTGTACCGCTGTGAGAAGTGCGACACCGA
  ATTTCCCAATTTCAAGTACCGCATGATCCTGTCAGTAAATATTGCAGATTTTCAAGAGAA
  TCAGTGGGTGACTTGTTTCCAGGAGTCTGCTGAAGCTATCCTTGGACAAAATGCTGCTTA
  TCTTGGGGAATTAAAAGACAAGAATGAACAGGCATTTGAAGAAGTTTTCCAGAATGCCAA
  CTTCCGATCTTTCATATTCAGAGTCAGGGTCAAAGTGGAGACCTACAACGACGAGTCTCG
  AATTAAGGCCACTGTGATGGACGTGAAGCCCGTGGACTACAGAGAGTATGGCCGAAGGCT
  GGTCATGAGCATCAGGAGAAGTGCATTGATGTGAGAGGAGCAGTGCCAATCGGGCAGAAG
  TTTGCAAATAGGCAGAATGGAATCGATTTCCTCCCACCTCCGTGTGACGATCCCATGTTA
  GCTACACAGTGCAGAGGCTCTTGATGGTGGACTAAGCAATTTCCTCCCTTGTGCGCATCT
  CAGAACCCATCGGTAGGCAAAGGAAAATACGCTCAGGTGGTTGTGGTGTAGACTGTGTCA
  GGCCTACGGAGTCAGCCAGTGGCTAGCGCAAGACCAGTCACTCCCTCTGCCTTCAGGCTT
  CTGTCAATTTCATTATCATCAAGCAGGAATTATGTCGTAAGTCACTGACCCTAACTGCAG
  ACCATGAAGTAAATTATGTAACTAGGTTTTTGCTTCTCCAGTGGTGACCACCCCCCCCCC
  ATCCCCGCTCACAACTTGGGTTCTTCTCAGCGGGGCGAGCTGAGAAGCGGTCATGAGCAC
  CTGGGGATTTTAGTAAGTGTGTCTTCCTAGAATTCGAAGGCTCTCTCTTTCTAGAGGTGC
  TACATAGTTGGTAATGCTTGGAATGGCAATAGGGTAGAATGATTAATCAAAGGCATATCT
  TCTATATCTGAAGAGTATCCTTCCTTCAGGGTTTAATAGACTGAGTCAGATGGGTCTGAT
  ATTAATCAAAATTGTCTCTTCTGAGGACCGCTGATAAGCATTGACTTGCTGTCCCCTAAG
  GAAATCCGAGCGGCTACAAAGCGTTTCTTTACTTTTCACTTCAATTAATGCTGCGCTTCG
  CTTGGTGAGTGCGTACTTTTTCTACCTGTACACATTCCTGCATTCATGTATTTTGTTTTT
  TTTGACTAAAGCTATGTTACATGGAAAGGATTTTGAAGCCTTTTGTTTCCCTTGCTTTGT
  TTTAATAAACAGTATATTCTTTGGTTGTGAATCCTAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAA
• Restriction Sites: Please inquire
• ACCN: NM_002945
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002945.2, NP_002936.1
• RefSeq Size: 2824 bp
• RefSeq ORF: 1851 bp
• Locus ID: 6117
• UniProt ID: P27694
• Cytogenetics: 17p13.3
• Domains: tRNA_anti, Rep-A_N
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: DNA replication, Homologous recombination, Mismatch repair, Nucleotide excision repair
• Gene Summary: This gene encodes the largest subunit of the heterotrimeric Replication Protein A (RPA) complex, which binds to single-stranded DNA (ssDNA), forming a nucleoprotein complex that plays an important role in DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance, and co-ordinating the cellular response to DNA damage through activation of the ataxia telangiectasia and Rad3-related protein (ATR) kinase. The nucleoprotein complex protects the single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. This subunit contains four oligonucleotide/oligosaccharide-binding (OB) domains, though the majority of ssDNA binding occurs in two of these domains. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which ssDNA binding domains are utilized. The different binding modes differ in the length of DNA bound and in the proteins with which it interacts, thereby playing a role in regulating different genomic maintenance pathways. [provided by RefSeq, Sep 2017]