NONO (NM_007363) Human Untagged Clone

CAT#: SC324587

NONO (untagged)-Human non-POU domain containing, octamer-binding (NONO), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NONO
• Synonyms: MRXS34; NMT55; NRB54; P54; P54NRB; PPP1R114
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_007363.3 GGAGAGGCCGTGTAGCGTCGCCGTTACTCCGAGGAGATACCAGTCGGTAGAGGAGAAGTC
  GAGGTTAGAGGGAACTGGGAGGCACTTTGCTGTCTGCAATCGAAGTTGAGGGTGCAAAAA
  TGCAGAGTAATAAAACTTTTAACTTGGAGAAGCAAAACCATACTCCAAGAAAGCATCATC
  AACATCACCACCAGCAGCAGCACCACCAGCAGCAACAGCAGCAGCCGCCACCACCGCCAA
  TACCTGCAAATGGGCAACAGGCCAGCAGCCAAAATGAAGGCTTGACTATTGACCTGAAGA
  ATTTTAGAAAACCAGGAGAGAAGACCTTCACCCAACGAAGCCGTCTTTTTGTGGGAAATC
  TTCCTCCCGACATCACTGAGGAAGAAATGAGGAAACTATTTGAGAAATATGGAAAGGCAG
  GCGAAGTCTTCATTCATAAGGATAAAGGATTTGGCTTTATCCGCTTGGAAACCCGAACCC
  TAGCGGAGATTGCCAAAGTGGAGCTGGACAATATGCCACTCCGTGGAAAGCAGCTGCGTG
  TGCGCTTTGCCTGCCATAGTGCATCCCTTACAGTTCGAAACCTTCCTCAGTATGTGTCCA
  ACGAACTGCTGGAAGAAGCCTTTTCTGTGTTTGGCCAGGTAGAGAGGGCTGTAGTCATTG
  TGGATGATCGAGGAAGGCCCTCAGGAAAAGGCATTGTTGAGTTCTCAGGGAAGCCAGCTG
  CTCGGAAAGCTCTGGACAGATGCAGTGAAGGCTCCTTCCTGCTAACCACATTTCCTCGTC
  CTGTGACTGTGGAGCCCATGGACCAGTTAGATGATGAAGAGGGACTTCCAGAGAAGCTGG
  TTATAAAAAACCAGCAATTTCACAAGGAACGAGAGCAGCCACCCAGATTTGCACAGCCTG
  GCTCCTTTGAGTATGAATATGCCATGCGCTGGAAGGCACTCATTGAGATGGAGAAGCAGC
  AGCAGGACCAAGTGGACCGCAACATCAAGGAGGCTCGTGAGAAGCTGGAGATGGAGATGG
  AAGCTGCACGCCATGAGCACCAGGTCATGCTAATGAGACAGGATTTGATGAGGCGCCAAG
  AAGAACTTCGGAGGATGGAAGAGCTGCACAACCAAGAGGTGCAAAAACGAAAGCAACTGG
  AGCTCAGGCAGGAGGAAGAGCGCAGGCGCCGTGAAGAAGAGATGCGGCGGCAGCAAGAAG
  AAATGATGCGGCGACAGCAGGAAGGATTCAAGGGAACCTTCCCTGATGCGAGAGAGCAGG
  AGATTCGGATGGGTCAGATGGCTATGGGAGGTGCTATGGGCATAAACAACAGAGGTGCCA
  TGCCCCCTGCTCCTGTGCCAGCTGGTACCCCAGCTCCTCCAGGACCTGCCACTATGATGC
  CGGATGGAACTTTGGGATTGACCCCACCAACAACTGAACGCTTTGGTCAGGCTGCTACAA
  TGGAAGGAATTGGGGCAATTGGTGGAACTCCTCCTGCATTCAACCGTGCAGCTCCTGGAG
  CTGAATTTGCCCCAAACAAACGTCGCCGATACTAATAAGTTGCAGTGTCTAGTTTCTCAA
  AACCCTTAAAAGAAGGACCCTTTTTGGACTAGCCAGAATTCTACCCTGGAAAAGTGTTAG
  GGATTCCTTCCAATAGTTAGATCTACCCTGCCTGTACTACTCTAGGGAGTATGCTGGAGG
  CAGAGGGCAAGGGAGGGGTGGTATTAAACAAGTCAATTCTGTGTGGTATATTGTTTAATC
  AGTTCTGTGTGGTGCATTCCTGAAGTCTCTAATGTGACTGTTGGGGGCCTGGGGAAACCA
  TGGCAAAGTGGATCCAGTTAGAGCCCATTAATCTTGATCATTCCGGTTTTTTTTTTTTTT
  TTGTCCATCTTGTTTCATTTGCTTGCCCCGCCCCCGAGACGGAGTCTTACTCTGTCGCCC
  AGGCTGGAGTGTAGTGGCATGATCTCGGCTCACTGCAATCTCTGCCTCCCGGGTTCAAGC
  TTGTCCAGGTTGATCTTGAACTCCTGACCTCGTGATCTACCCACCTCGGCCTCCCAAAAT
  GCTGGGATTACAGGGGTGAGCCACCGTGCCCAACCTCACTTGCTTCTTATCCTTACACTC
  CCCCAGCCCCAGAGAAACTGCCACATACACCACAAAAACCAAACATCCCCCAATGACCTT
  AGCCCCATTGCTCCATTCACTCCCAGGTGAGAATTCAGGCAAACGTCCACAAAGGTCACA
  GGCAGCGTACATACGGTTCTGTTATACCCCATATATTACCCCTTCATGTCCTAAAGAAGA
  CATTTTCTCTTAGAGATTTTCATTTTAGTGTATCTTTAAAAAAAAATCTTGTGTTAACTT
  GCCTCCATCTTTTTCTTGGGTGAGGACACCCAGGAATGACCCTTTTGTGTCTATGATGTT
  GCTGTTCACAGCTTTTCTTGATAGGCCTAGTACAATCTTGGGAACAGGGTTACTGTATAC
  TGAAGGTCTGACAGTAGCTCTTAGACTCGCCTATCTTAGGTAGTCATGCTGTGCATTTTT
  TTTTTTCATTGGTGTACTGTGTTTGATTTGTCTCATATATTTGGAGTTTTTCTGAAAAAT
  GGAGCAGTAATGCAGCATCAACCTATTAAAATACATTTTAAGCCTTTTAAAAAAAAAAAA
  AAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_007363
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007363.3, NP_031389.3
• RefSeq Size: 2705 bp
• RefSeq ORF: 1416 bp
• Locus ID: 4841
• UniProt ID: Q15233
• Cytogenetics: Xq13.1
• Domains: RRM
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16. [provided by RefSeq, Feb 2009]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein (isoform 1).